Background

Titanium dioxide (TiO₂) is employed in the production of cosmetics, sunscreen, food, and drugs; however, TiO₂ is toxic at the nanometric scale.

Objective

To analyze the in vitro toxic effect of TiO₂ NPs on mesenchymal stem cells (hMSCs).

Methods

The hMSCs from Wharton`s jelly were exposed to 4, 62.5 and 500 μg/mL of TiO₂ NPs. Viability and cell proliferation tests were carried out at 2, 5 and 7 days of exposure. Additionally, the osteogenic and adipogenic differentiation capacity was evaluated.

Results

TiO₂ NPs had a perinuclear arrangement and internalized in vesicles into cytosol, diminished significantly the viability (40% and 30% and 30%) and the proliferation cellular (35%, 50% and 55%), differentiation to osteoblast (38%) and adipoblasts (20%).

Conclusion

TiO₂ NPs affect viability, proliferation, and differentiation of hMSCs. Our result suggests more regulation of the use of TiO_2.

中文翻译：

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二氧化钛纳米粒子对人间充质干细胞的毒性作用

背景

二氧化钛（TiO ₂）用于生产化妆品，防晒霜，食品和药品；然而，TiO ₂在纳米尺度上是有毒的。

目的

分析TiO ₂ NPs对间充质干细胞（hMSCs）的体外毒性作用。

方法

将来自沃顿商学院果冻的hMSC暴露于4、62.5和500μg/ mL的TiO ₂ NP中。在暴露后2、5和7天进行生存力和细胞增殖测试。另外，评估了成骨和成脂分化能力。

结果

TiO ₂ NP具有核周排列，并在囊泡中内化为细胞溶质，显着降低了活力（40％，30％和30％）和增殖细胞（35％，50％和55％），分化为成骨细胞（38％）和成脂细胞（20％）。

结论

TiO ₂ NPs影响hMSC的活力，增殖和分化。我们的结果表明对TiO ₂使用的更多规定_。