We analyzed by bidimensional electrophoresis the acid-insoluble fraction of saliva from three classes of angioedema patients and a healthy control group, highlighting significant variations of several normalized spot volumes. Characterization of the corresponding proteins was performed by in-gel tryptic digestion of the spots, followed by high-resolution HPLC-ESI-MS/MS analysis of tryptic mixtures. By this strategy, 16 differentially-expressed proteins among two or more groups were identified. We found higher concentration of proteins involved in immune response (interleukin-1 receptor antagonist and annexin A1), and of moonlighting proteins acting as plasminogen receptors (glyceraldehyde-3-phosphate dehydrogenase, α-enolase, and annexin A2) in patients affected by the idiopathic non-histaminergic or hereditary angioedema with unknown origin with respect to healthy controls. These data provide new information on the molecular basis of these less characterized types of angioedema.

Graphical Abstract

我们通过二维电泳分析了来自三类血管性水肿患者和一个健康对照组的唾液酸不溶性部分，突出了几个标准化斑点体积的显着变化。通过对斑点进行凝胶内胰蛋白酶消化，然后对胰蛋白酶混合物进行高分辨率 HPLC-ESI-MS/MS 分析，对相应蛋白质进行表征。通过这种策略，鉴定了两组或更多组之间的 16 种差异表达蛋白质。我们发现参与免疫反应的蛋白质浓度更高（白细胞介素 1 受体拮抗剂和膜联蛋白 A1），以及充当纤溶酶原受体的月光蛋白（3-磷酸甘油醛脱氢酶、α-烯醇化酶、和膜联蛋白 A2) 在受特发性非组胺能或遗传性血管性水肿影响的患者中，与健康对照相比来源不明。这些数据提供了关于这些特征较少的血管性水肿类型的分子基础的新信息。

图形概要