This study proposes an innovative strategy of lignocellulose biodegradation by Inonotus obliquus under solid-state fermentation in extracting Eucommia ulmoides trans-1,4-polyisoprene (EUG) and producing reducing sugars efficiently. EUG and sugars were obtained through the white rot fungal pretreatment of E. ulmoides leaves, ultrasound-assisted solvent extraction, and enzymatic saccharification. After mere 2-day fermentation, the loss of lignin, cellulose, and hemicelluloses of the leaves achieved 7.11%, 3.47%, and 6.44%, respectively due to the high activity levels of manganese peroxidase (MnP, 973 IU g⁻¹) and lignin peroxidase (LiP, 1341 IU g⁻¹) produced by the fungus. The breakdown of fibrous networks brought higher yields of EUG and reducing sugars. The highest extraction yield of EUG was 4.86% from the 2-day fermented leaves, 31.4% greater than that from the control (3.69%). Meanwhile, the leaf residues after EUG extraction released 97.8 mg g⁻¹ reducing sugars with enzymatic saccharification, 77.5% greater than that from the control (55.1 mg g⁻¹). The results demonstrated that I. obliquus could use E. ulmoides leaves as substrate to produce high-activity-level ligninolytic enzymes in a very short time and the lignocellulose selective degradation of E. ulmoides leaves enhanced the yields of EUG and reducing sugars.

这项研究提出了一种创新的策略，即在固态发酵条件下，用斜切线虫（Inonotus obliquus）降解木糖，以提取杜仲杜仲反式1,4-聚异戊二烯（EUG）并有效地生产还原糖。EUG和糖是通过白色腐霉真菌（E. ulmoides）叶片的预处理，超声辅助溶剂提取和酶促糖化作用而获得的。经过仅两天的发酵，由于锰过氧化物酶（MnP，973 IU g ^-1）和高活性水平，叶片木质素，纤维素和半纤维素的损失分别达到7.11％，3.47％和6.44％。木质素过氧化物酶（LiP，1341 IU g ^-1）由真菌产生。纤维网络的破裂带来了较高的EUG产量和还原糖。2天发酵叶的EUG提取率最高，为4.86％，比对照（3.69％）高31.4％。同时，EUG提取后的叶片残留物经酶促糖化释放出97.8 mg g ^-1还原糖，比对照的55.1 mg g ^-1高77.5％。结果表明，I.斜可以使用杜仲的叶子作为底物，以产生在一个非常短的时间高活性级木质素降解酶和木质纤维素选择性降解杜仲增强EUG和还原糖的产率叶子。