Vanilla planifolia (V. planifolia) is a valuable orchidaceous plant, commonly grown for its pods that are used to produce the flavouring vanilla extract. Here, we evaluated the effect of calcium lignosulphonate (Ca-LIGN) and sodium lignosulphonate (Na-LIGN) on multiplication and regeneration of V. planifolia shoot tip culture. In 150 mg L⁻¹ Ca-LIGN medium, the most number of shoots per explant (5.78 ± 0.63) was successfully obtained. Besides, Ca-LIGN also enhanced the shoot bud and primordial formation rate, as seen under scanning electron microscopy. In contrast, medium containing 150 mg L⁻¹ Na-LIGN recorded the highest average of shoot length (4.72 ± 0.30 cm). Meanwhile, the best growth of root length (1.8 ± 0.32 cm) and root induction (96.67 ± 5.16%) were recorded on the explants treated with 150 mg L⁻¹ Na-LIGN rooting medium. All rooted plantlets successfully acclimatized in the greenhouse (100.00% survival rate). Further biochemical analysis revealed that Ca-LIGN increased the total protein, chlorophyll, sugar, flavonoid and phenolic contents of V. planifolia. Notably, expression of both ribulose-1,5-bisphosphate carboxylase (Rubisco) and phosphoenolpyruvate carboxylase (PEPC) genes were also elevated under the treatment of Ca-LIGN, implying a positive role in the photosynthetic process. Taken together, LIGN being an environmental friendly product could be used to enhance the growth and micropropagation of V. planifolia.

香草planifolia（V. planifolia）是一种有价值的兰花植物，通常为其荚果而种植，用于生产调味香草提取物。在这里，我们评估了木素磺酸钙（CA-河旁）和木质素钠（Na-河旁）对乘法和再生的效果V.荚兰茎尖培养。在150 mg L ^-1 Ca-LIGN培养基中，成功获得了每个外植体最多的芽数（5.78±0.63）。此外，如在扫描电子显微镜下所见，Ca-LIGN还提高了芽的芽和原始的形成速率。相反，含有150 mg L ^-1的培养基Na-LIGN记录的平均芽长最高（4.72±0.30 cm）。同时，在用150 mg L ^-1 Na-LIGN生根培养基处理过的外植体上，根长最佳（1.8±0.32 cm）和根诱导最佳（96.67±5.16％）。所有生根的小苗均已成功适应温室（成活率为100.00％）。进一步的生化分析显示，钙河旁增加的总蛋白质，叶绿素，糖，黄酮类化合物和酚含量V.荚兰。值得注意的是，核糖-1,5-双磷酸羧化酶（Rubisco）和磷酸烯醇丙酮酸羧化酶（PEPC）的表达）基因在Ca-LIGN处理下也升高，暗示在光合作用过程中发挥积极作用。综上所述，LIGN是一种环境友好的产品，可以用来促进V. Planifolia的生长和微繁殖。