A multifunctional targeted nanoplatform combining photothermal therapy and chemotherapy has emerged as a promising strategy for comprehensive therapies of breast cancer. In this study, we constructed human epidermal growth factor receptor 2 (Her2)-targeted gold nanoshelled poly(lactic-co-glycolic acid) hybrid nanocapsules encapsulating perfluorooctyl bromide, superparamagnetic iron oxide nanoparticles, and doxorubicin (Her2-GPDH nanocapsules) as theranostic agent for bimodal ultrasound/magnetic resonance imaging and synergistic photothermal-chemotherapy of Her2-postive breast cancer cells. Her2–GPDH nanocomposites possessed well-defined spherical morphology, and the average diameter was about 296 nm with good dispersion. Targeting assays demonstrated that Her2–GPDH nanocapsules exhibited higher targeting binding to Her2-positive SKBR3 cells than Her2-negative MDA-MB-231cells. The encapsulation efficiency and the loading content of doxorubicin in Her2–GPDH nanocapsules were 39 ± 1.45% and 3.8 ± 0.52%, respectively, and the agent exhibited pH-responsive and near-infrared light-triggered stepwise release behavior of doxorubicin. In vitro, the agent had potential to serve as feasible candidate for ultrasound imaging and T₂-weighted magnetic resonance imaging with a relatively high relaxivity. Cell experiments confirmed that the agent had significant photothermal cytotoxicity on SKBR3 cells, and the combined photothermal–chemotherapy could significantly enhance the anti-tumor effect. In summary, the present Her2–GPDH nanocapsules, a novel multifunctional nanoplatform, will offer a new way for early bimodal molecular-level diagnosis and synergistic treatment of Her2-positve breast cancer.

结合光热疗法和化学疗法的多功能靶向纳米平台已成为乳腺癌综合治疗的有前途的策略。在这项研究中，我们构建了人表皮生长因子受体2（HER2）靶向性金nanoshelled聚（乳酸-共-乙醇酸）混合纳米胶囊，包裹全氟辛基溴、超顺磁性氧化铁纳米颗粒和多柔比星（Her2-GPDH 纳米胶囊），作为双峰超声/磁共振成像和协同光热化疗对 Her2 阳性乳腺癌细胞的治疗诊断剂。Her2-GPDH 纳米复合材料具有明确的球形形态，平均直径约为 296 nm，具有良好的分散性。靶向测定表明，与 Her2 阴性 MDA-MB-231 细胞相比，Her2-GPDH 纳米胶囊对 Her2 阳性 SKBR3 细胞具有更高的靶向结合。Her2-GPDH纳米胶囊中阿霉素的包封率和负载量分别为39±1.45%和3.8±0.52%，该药物表现出pH响应性和近红外光触发的阿霉素逐步释放行为。具有相对高弛豫率的₂加权磁共振成像。细胞实验证实该药剂对SKBR3细胞具有显着的光热细胞毒性，光热联合化疗可显着增强抗肿瘤作用。总之，目前的 Her2-GPDH 纳米胶囊是一种新型多功能纳米平台，将为 Her2 阳性乳腺癌的早期双峰分子水平诊断和协同治疗提供新方法。