Lumpy skin disease and Rift Valley fever are two high-priority livestock diseases which have the potential to spread into previously free regions through animal movement and/or vectors, as well as intentional release by bioterrorists. Since the distribution range of both diseases is similar in Africa, it makes sense to use a bivalent vaccine to control them. This may lead to the more consistent and sustainable use of vaccination against Rift Valley fever through a more cost-effective vaccine. In this study, a recombinant lumpy skin disease virus was constructed in which the thymidine kinase gene was used as the insertion site for the Gn and Gc protective glycoprotein genes of Rift Valley fever virus using homologous recombination. Selection markers, the enhanced green fluorescent protein and Escherichia coli guanidine phosphoribosyl transferase (gpt), were used for selection of recombinant virus and in a manner enabling a second recombination event to occur upon removal of the gpt selection-pressure allowing the removal of both marker genes in the final product. This recombinant virus, LSD-RVF.mf, was selected to homogeneity, characterized and evaluated in cattle as a vaccine to show protection against both lumpy skin disease and Rift Valley fever in cattle. The results demonstrate that the LSD-RVF.mf is safe, immunogenic and can protect cattle against both diseases.

中文翻译：

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使用二价块状皮肤病病毒载体重组裂谷热疫苗接种牛的保护。

块状皮肤病和裂谷热是两种高度优先的牲畜疾病，它们有可能通过动物运动和/或媒介传播以及生物恐怖分子有意释放而传播到以前的自由地区。由于两种疾病在非洲的分布范围相似，因此使用二价疫苗控制它们是有意义的。通过更具成本效益的疫苗，这可能导致针对裂谷热的疫苗更加一致和可持续地使用。在这项研究中，构建了重组块状皮肤病病毒，其中使用胸腺嘧啶激酶基因作为裂谷热病毒的Gn和Gc保护性糖蛋白基因的同源重组插入位点。选择标记 使用增强的绿色荧光蛋白和大肠杆菌胍基磷酸核糖基转移酶（gpt）来选择重组病毒，并以能够在去除gpt选择压力时发生第二次重组事件的方式进行，从而可以去除gpt中的两个标记基因。完成品。选择该重组病毒LSD-RVF.mf，使其具有同质性，在牛中进行表征和评估，以作为疫苗显示出对牛的块状皮肤病和裂谷热的保护。结果表明，LSD-RVF.mf是安全的，具有免疫原性并且可以保护牛免受两种疾病的侵害。选择该重组病毒LSD-RVF.mf，使其具有同质性，在牛中进行表征和评估，以作为疫苗显示出对牛的块状皮肤病和裂谷热的保护。结果表明，LSD-RVF.mf是安全的，具有免疫原性并且可以保护牛免受两种疾病的侵害。选择该重组病毒LSD-RVF.mf，使其具有同质性，在牛中进行表征和评估，以作为疫苗显示出对牛的块状皮肤病和裂谷热的保护。结果表明，LSD-RVF.mf是安全的，具有免疫原性并且可以保护牛免受两种疾病的侵害。