Nitraria tangutorum B. (NT), Hippophae rhamnoides L. (HR), Lycium ruthenicum M. (LR), Lycii fructus (LF), Rosa xanthina L. (RX), and Rubuscor chorifolius L. f. (RC) are six berries from Tibetan Plateau. They have been used in traditional folk medicine with a long history, which are rich in anthocyanins. However, detailed study of their anthocyanins remains scarce. Therefore, a method for rapid simultaneous identification and quantification of 12 anthocyanins from berries using UPLC-Quadruple-Orbitrap MS system (UPLC-Q-Orbitrap MS) was established in this work. It was verified with limit of detection (3.86–11.61 µg/L), limit of quantification (3.86–11.61 µg/L), precision (0.95–2.38%), repeatability (0.96–2.08%), stability (0.86–2.31%), mean recovery (95.8–103.1%), recovery range (93.1–107.2%) and RSD less than 5.21%. It was then used in the analysis of anthocyanins in six berries species; 8, 7, 7, 7, 6 and 9 species of anthocyanins have been identified in NT, LF, LR, HR, RC and RX, respectively based on their own retention time and exact mass in positive mode, and for the first time quantified successfully in each berry (31.11 ± 0.42–2978 ± 25.67 μg.g−1). Finally, 2, 2-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging activity (0.92 ± 0.12–5.61 ± 0.23 mM TE/100 g), ferric reducing antioxidant power (FRAP) (1.23 ± 0.15–7.42 ± 0.28 mM TE/100 g) and total antioxidant activity (T-AOC) assays were used to evaluate the antioxidant activities of the six berries.

中文翻译：

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UPLC-quadruple-Orbitrap MS快速定性定量分析青藏高原浆果中的花青素成分及其抗氧化活性

Nitraria tangutorum B. (NT)、Hippophae rhamnoides L. (HR)、Lycium ruthenicum M. (LR)、Lycii fructus (LF)、Rosa xanthina L. (RX) 和 Rubuscor chorifolius L. f. (RC) 是来自青藏高原的六种浆果。它们被用于传统的民间医学，历史悠久，富含花青素。然而，对其花青素的详细研究仍然很少。因此，本工作建立了一种使用 UPLC-Quadruple-Orbitrap MS 系统（UPLC-Q-Orbitrap MS）快速同时鉴定和定量浆果中 12 种花青素的方法。通过检测限 (3.86–11.61 µg/L)、定量限 (3.86–11.61 µg/L)、精密度 (0.95–2.38%)、重复性 (0.96–2.08%)、稳定性 (0.86–2.31%) 进行验证)、平均回收率 (95.8–103.1%)、回收率范围 (93.1–107.2%) 和 RSD 小于 5.21%。然后将其用于分析六种浆果中的花青素；8、7、7、7、6和9种花青素在NT、LF、LR、HR、RC和RX中分别基于其保留时间和正离子模式下的精确质量被鉴定，并首次定量在每个浆果中成功（31.11 ± 0.42–2978 ± 25.67 μg.g-1）。最后，2, 2-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) 自由基清除活性 (0.92 ± 0.12–5.61 ± 0.23 mM TE/100 g)，铁还原抗氧化能力 (FRAP) (1.23 ± 0.15– 7.42 ± 0.28 mM TE/100 g) 和总抗氧化活性 (T-AOC) 测定用于评估六种浆果的抗氧化活性。分别基于它们自己的保留时间和正模式下的精确质量，并首次在每个浆果中成功定量 (31.11 ± 0.42–2978 ± 25.67 μg.g-1)。最后，2, 2-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) 自由基清除活性 (0.92 ± 0.12–5.61 ± 0.23 mM TE/100 g)，铁还原抗氧化能力 (FRAP) (1.23 ± 0.15– 7.42 ± 0.28 mM TE/100 g) 和总抗氧化活性 (T-AOC) 测定用于评估六种浆果的抗氧化活性。分别基于它们自己的保留时间和正模式下的精确质量，并首次在每个浆果中成功定量 (31.11 ± 0.42–2978 ± 25.67 μg.g-1)。最后，2, 2-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) 自由基清除活性 (0.92 ± 0.12–5.61 ± 0.23 mM TE/100 g)，铁还原抗氧化能力 (FRAP) (1.23 ± 0.15– 7.42 ± 0.28 mM TE/100 g) 和总抗氧化活性 (T-AOC) 测定用于评估六种浆果的抗氧化活性。