In this work, we studied the purification with ion exchange chromatography of an aminoglycoside antibiotic industrially produced by Fareva. To do so, we performed a set of well targeted experiments and developed a physical model properly describing the ion exchange mechanism and acid-base equilibria in solution using the simulation software Ypso-Ionic. Our approach allowed us to rationalize few intriguing observations like the unexpectedly low capacity of the resin for the antibiotic or the strong influence of the counterion on the separation of two isomers of the antibiotic. Our findings illustrate the strength of using rigorous ion exchange models – as opposed to the often used classical adsorption models – to describe ion exchange chromatography.

在这项工作中，我们研究了用离子交换色谱法纯化Fareva工业生产的氨基糖苷类抗生素。为此，我们进行了一系列针对性强的实验，并开发了一个物理模型，使用模拟软件Ypso-Ionic正确描述了溶液中的离子交换机理和酸碱平衡。我们的方法使我们能够合理化一些有趣的观察，例如树脂对抗生素的出乎意料的低容量或抗衡离子对抗生素的两种异构体分离的强烈影响。我们的发现说明了使用严格的离子交换模型（与通常使用的经典吸附模型相对）来描述离子交换色谱的优势。