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Atomic oxygen radical‐induced intracellular oxidization of mould spore cells
Plasma Processes and Polymers ( IF 3.5 ) Pub Date : 2020-06-08 , DOI: 10.1002/ppap.202000001
Yuta Tanaka 1 , Jun‐Seok Oh 2 , Hiroshi Hashizume 3 , Takayuki Ohta 1 , Masashi Kato 4 , Masaru Hori 3 , Masafumi Ito 1
Affiliation  

The inactivation mechanism of mould spores using plasma‐generated neutral reactive oxygen species (ROS) was investigated in this paper. Typical cell viability using a counting of colony‐forming unit assay indicated a major state of the spore cells. Both intracellular and extracellular damages have been investigated by a couple of well‐established cell visualization techniques: confocal fluorescence emission microscopy, scanning electron microscopy, and transmission electron microscopy. It is revealed in this paper that the combination of the spore cell viability with those visualization results strongly suggested an early stage of the cell inactivation when ROS can induce intracellular lipid peroxidation through both nanometer‐thick lipid cell membrane and less‐damaged several hundred nanometer‐thick cell wall.

中文翻译:

原子氧自由基诱导的霉菌孢子细胞胞内氧化

本文研究了等离子体产生的中性活性氧(ROS)对霉菌孢子的失活机理。使用计数菌落形成单位测定法的典型细胞活力表明了孢子细胞的主要状态。细胞内和细胞外损伤均已通过几种成熟的细胞可视化技术进行了研究:共聚焦荧光发射显微镜,扫描电子显微镜和透射电子显微镜。本文揭示了孢子细胞活力与这些可视化结果的结合强烈表明,当ROS可以通过纳米厚的脂质细胞膜诱导细胞内脂质过氧化并且损伤较少的几百纳米时,ROS可以处于细胞失活的早期阶段。厚的细胞壁。
更新日期:2020-06-08
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