In order to run a series of in vitro studies on the effect of extremely low‐frequency magnetic fields on cell cultures, developing and characterizing an appropriate exposure system is required. The present design is based on a two‐shielded Lee‐Whiting coils system. The circular design was chosen because its axial symmetry allowed for both reducing simulation unknowns and measurement points during the characterization, and additionally made the machining of the parts easier. The system can generate magnetic flux densities (B fields) up to 1 mT root‐mean‐square amplitude (rms) with no active cooling system in the incubator, and up to 3 mT_rms with it. The double‐wrapped windings with twisted pairs allow for the use of each set of coils either as exposure or control with no detectable parasitic B field in the control. The artifacts have also been analyzed; the B field in the center of the sham control chamber is about 1 µT_rms for a maximum of 3 mT_rms in the exposure chamber, the parasitic incident electric fields are less than 1 V/m, the temperature difference between sham and exposure chamber is less than or equal to 0.2 °C, and the typical vibration difference between sham and exposure is less than 0.1 m/s². © 2020 Bioelectromagnetics Society.

中文翻译：

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基于Lee-Whiting线圈的ELF体外研究磁场暴露系统。

为了对极低频磁场对细胞培养的影响进行一系列体外研究，需要开发和表征合适的曝光系统。本设计基于两屏蔽式Lee-Whiting线圈系统。选择圆形设计是因为其轴向对称性可减少特征描述期间的模拟未知数和测量点，并且使零件的加工更加容易。该系统在培养箱中不使用主动冷却系统的情况下，可以产生高达1 mT的均方根振幅（rms）的磁通密度（B场），并且高达3 mT _rms用它。带有双绞线的双缠绕绕组允许将每组线圈用作曝光或控制，而控制中没有可检测到的寄生B场。还对文物进行了分析；伪控制室中心的B场约为1 µT _rms，曝光室中的最大值为3 mT _rms，寄生入射电场小于1 V / m，伪影与曝光室之间的温差为小于或等于0.2°C，并且假冒和曝光之间的典型振动差小于0.1 m / s ²。©2020生物电磁学会。