Enzyme immobilization enhances the catalytic activity and stability of the enzyme, and also improves reusability. Metal–organic frameworks (MOFs), which possess diversified structures and porosity, have been used as excellent carriers for enzyme immobilization. Pseudomonas fluorescens lipase (PFL) has been successfully immobilized onto MOFs by covalent cross‐linking to obtain a series of immobilized lipase (PFL@MOFs). PFL@MOFs are used for catalytic enantioselective hydrolysis of 2‐(4‐hydroxyphenyl) propionic acid ethyl ester enantiomers (2‐HPPAEE) in aqueous medium and transesterification of 4‐methoxymandelic acid enantiomers (4‐MMA) in organic medium. The experimental results indicated that PFL@Uio‐66(Zr) exhibits excellent enzymatic catalysis performances and high enantioselectives. In addition, to improve catalytic activity and reusability, PFL is modified by the polyethylene glycol (PEG) to prepare PEG‐modified lipase (PFL‐PEG), then PFL‐PEG is immobilized onto Uio‐66(Zr) to prepare PFL‐PEG@Uio‐66(Zr), demonstrating better reusability and catalytic activity compared with PFL@Uio‐66(Zr).

中文翻译：

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将脂肪酶固定在金属有机骨架上以进行对映选择性水解和酯交换

固定酶可以增强酶的催化活性和稳定性，还可以提高可重复使用性。具有多种结构和孔隙率的金属有机骨架（MOF）已被用作固定化酶的优良载体。荧光假单胞菌脂肪酶（PFL）已通过共价交联成功固定在MOF上，从而获得了一系列固定化脂肪酶（PFL @ MOFs）。PFL @ MOF用于在水性介质中催化2-（4-羟基苯基）丙酸乙酯对映体（2-HPPAEE）的催化对映选择性水解和在有机介质中对4-甲氧基扁桃酸对映体（4-MMA）的酯交换反应。实验结果表明，PFL @ Uio-66（Zr）具有优异的酶催化性能和高对映选择性。此外，为提高催化活性和可重复使用性，可通过聚乙二醇（PEG）修饰PFL以制备PEG修饰的脂肪酶（PFL-PEG），然后将PFL-PEG固定在Uio-66（Zr）上以制备PFL-PEG @ Uio-66（Zr），与PFL @ Uio-66（Zr）相比，具有更好的重用性和催化活性。