We described the ectomycorrhizae (ECM) of the Japanese Lactarius section Deliciosi species L. akahatsu, L. hatsudake, L. laeticolor, and L. subindigo with the aim of promoting the use of mycorrhizae for taxonomy and expansion of available cultures. Lactarius tottoriensis (sect. Lactarius) was also included as a comparator. The ECM of the four species of sect. Deliciosi showed orange laticifers and plectenchymatous mantles, whereas those of L. tottoriensis showed white laticifers and pseudoparenchymatous mantles. The ECM of L. hatsudake and L. subindigo showed complex rhizomorphs with thicker walls and more developed vessel-like hyphae compared to the other three species. Cystidium-like cells were rarely found on the mantles of L. laeticolor and L. subindigo. Fungal cultures of the five Lactarius species from their ECM were morphologically characterized. Cultures produced laticifers on agar medium, but the five species showed different growth rates. Rhizomorphs of L. hatsudake and L. subindigo in cultures showed a similar morphology to their ECM. Molecular phylogenetic analyses of cultures from the ECM demonstrated that they were of the targeted Lactarius species and suggested the need for taxonomic studies of L. hatsudake, L. subindigo, and L. tottoriensis at the species and section levels.

我们描述了日本乳杆菌科Deliciosi物种L的外生菌根（ECM）。akahatsu，大号。Hatsudake，L。laeticolor，和大号。subindigo旨在促进菌根用于分类学和扩展可用文化。乳tottoriensis（第乳）也包括作为比较器。四个教派的ECM。Deliciosi表现出橙色的胶乳化石和褶状披风，而tottoriensis呈白色乳胶状和假薄壁披风。初产的L. hatsudake和L的ECM 。与其他三个物种相比，亚靛蓝显示出复杂的根形，壁较厚，容器样菌丝更发达。在L的地幔中很少见到囊状细胞。laeticolor和大号。亚靛蓝。从形态学上表征了来自其ECM的五个乳杆菌属的真菌培养物。培养物在琼脂培养基上产生了乳杆菌，但是这五个物种显示出不同的生长速率。的Rhizomorphs大号。菇和大号。亚靛蓝在文化中显示出与他们的ECM相似的形态。来自ECM的培养物的分子系统发育分析表明，它们是靶向乳杆菌的物种，并提示需要对L. hatsudake，L .进行分类学研究。subindigo和L. tottoriensis在物种和剖面层次上。