Sets of multiple biomarkers can be applied to achieve a comprehensive assessment of the biological impact of the chemical pollutants in contaminated soils. This approach requires the integration of diverse biomarker data into indices that provide early signs of ecosystem health disturbance. Within this context, this study aims at integrating multiple biomarkers in green garden snail, Cantareus apertus, on the basis of data obtained in preceding laboratory and field studies. In the laboratory, snails had been treated with dietary Pb, Cd and their mixture. Prompt (1 wk) and long-lasting (8 wk) response profiles were characterized and the biomarkers integrated as Integrated Biomarker Response (IBR/n) index. The response profiles were dose and treatment time dependent in quantitative terms and also qualitatively different along the treatment time. Yet, IBR/n was sensitive to provide early (1 wk) signs of health disturbance caused by the metals, and remained sensitive in the long term (8 wk). In the field study, soil metal pollution was investigated along a distance gradient from a main roadside. Both metal levels in the soil–plant-snail system and a set of biomarkers in snails were recorded. The IBR/n revealed that disturbance in soil ecosystem health was restricted to short distances (at 20 m but not at 150 m). Therefore, IBR/n was a suitable approach also in the complexity of the field. Moreover, together with the IBR/n index, recently developed indices of bioavailability (SET) and risk (ERITME) were applied in the field study. In accordance with the IBR/n index, the SET index also revealed an excess transfer of metals to snails limited to a short distance from the roadside and identified Cd as the priority target for soil pollution management. Likewise, the ERITME classification rank confirmed that environmental risk was the highest at 20 m away from the roadside and then decreased at 150 m and beyond. Therefore, the application of the IBR/n index together with existing indices of the bioavailability (excess transfer) and risk of contaminants represents a challenging approach for the integrated environmental risk assessment of contaminated soils.

可以使用多种生物标志物的集合来对污染土壤中化学污染物的生物影响进行全面评估。这种方法需要将各种生物标记数据整合到提供生态系统健康扰乱早期迹象的指标中。在此背景下，本研究旨在将多个生物标记整合到绿色蜗牛中，即Cantareus apertus。，基于先前实验室和现场研究获得的数据。在实验室中，蜗牛已通过饮食中的Pb，Cd及其混合物进行了治疗。表征了快速反应（1周）和持久反应（8周），并将生物标志物整合为综合生物标志物响应（IBR / n）指数。反应曲线在剂量和剂量上取决于剂量和治疗时间，并且沿治疗时间在质量上也有所不同。然而，IBR / n很敏感，可以提供早期（1周）由金属引起的健康干扰迹象，并且长期（8周）仍然敏感。在野外研究中，沿着距主要路边的距离梯度调查了土壤金属污染。记录了土壤-植物-蜗牛系统中的金属含量以及蜗牛中的一组生物标记物。IBR / n揭示了土壤生态系统健康受到的干扰仅限于短距离（20 m处但不是150 m处）。因此，在该领域的复杂性中，IBR / n也是一种合适的方法。此外，连同IBR / n指数，最近开发的生物利用度（SET）和风险（ERITME）指数已应用于现场研究。根据IBR / n指数，SET指数还显示出金属向蜗牛的过量转移，仅限于距路边不远的地方，并将Cd确定为土壤污染管理的优先目标。同样，ERITME分类等级也证实，环境风险在距路边20 m处最高，而在150 m及以后处降低。因此，