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Baicalin attenuates endometritis in a rabbit model induced by infection with Escherichia coli and Staphylococcus aureus via NF-κB and JNK signaling pathways.
Domestic Animal Endocrinology ( IF 2.1 ) Pub Date : 2020-06-09 , DOI: 10.1016/j.domaniend.2020.106508
Y Miao 1 , M Ishfaq 1 , Y Liu 1 , Z Wu 1 , J Wang 1 , R Li 1 , F Qian 1 , L Ding 2 , J Li 1
Affiliation  

In this study, a rabbit endometritis model was developed to study cow endometritis. In addition, the protective effects of baicalin (a flavonoid) against endometritis were investigated. Clinical symptoms, differential leukocyte counting, uterine secretion smear microscopy and chemical examination, urine testing, and signs of necropsy showed abnormal changes and inflammatory responses in the uterus of rabbits. Histopathological results revealed visible inflammatory exudates and blood spots between intercellular spaces which confirmed that the rabbit endometritis model was successfully developed. Most importantly, these inflammatory signs were partially attenuated with baicalin treatment. The data revealed that the increased body temperature and leukocyte cells, pus, and the detachment of epithelial cells were alleviated with baicalin administration in a dose-dependent manner. Histopathological tissue changes such as inflammatory cells infiltrates, hyperemia, hemorrhages, and shedding of epithelial cells were partially attenuated with baicalin treatment. In addition, the mRNA expression of inflammation-related genes (iNOS, IL-1β, TNF-α, IL-10, IL-4, and IL-6) was significantly altered in RAW264.7 cells after LPS treatment. Further, the phosphorylated protein expression of JNK, p65, and IκBα were significantly reduced with LPS treatment. Intriguingly, baicalin pretreatment reversed the alteration in mRNA expression of inflammation-related genes and significantly reduced the phosphorylation of JNK, p65, and IκBα. In summary, our results suggest that baicalin has protective effects on bacterial-induced endometritis in rabbits that involve the suppression of NF-κB and JNK signaling pathways and pro-inflammatory cytokines.



中文翻译:

黄芩苷通过 NF-κB 和 JNK 信号通路减轻由大肠杆菌和金黄色葡萄球菌感染诱导的兔模型的子宫内膜炎。

在这项研究中,开发了兔子宫内膜炎模型来研究奶牛子宫内膜炎。此外,研究了黄芩苷(一种黄酮类化合物)对子宫内膜炎的保护作用。临床症状、白细胞分类计数、子宫分泌物涂片镜检及化学检查、尿检及尸检体征均显示家兔子宫有异常变化及炎症反应。组织病理结果显示细胞间隙可见炎性渗出物和血斑,证实兔子宫内膜炎模型建立成功。最重要的是,黄芩苷治疗部分减轻了这些炎症迹象。数据显示,体温升高和白细胞、脓、黄芩苷以剂量依赖性方式减轻上皮细胞的脱落。黄芩苷治疗可部分减轻炎性细胞浸润、充血、出血和上皮细胞脱落等组织病理学组织变化。此外,LPS 处理后 RAW264.7 细胞中炎症相关基因(iNOS、IL-1β、TNF-α、IL-10、IL-4 和 IL-6)的 mRNA 表达显着改变。此外,LPS 处理显着降低了 JNK、p65 和 IκBα 的磷酸化蛋白表达。有趣的是,黄芩苷预处理逆转了炎症相关基因 mRNA 表达的改变,并显着降低了 JNK、p65 和 IκBα 的磷酸化。总之,

更新日期:2020-06-09
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