当前位置: X-MOL 学术Sci. China Chem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Bioactive polypeptide hydrogels modified with RGD and N-cadherin mimetic peptide promote chondrogenic differentiation of bone marrow mesenchymal stem cells
Science China Chemistry ( IF 9.6 ) Pub Date : 2020-06-08 , DOI: 10.1007/s11426-020-9772-0
Yan Rong , Zhen Zhang , Chaoliang He , Xuesi Chen

Cell-material and cell-cell interactions represent two crucial aspects of the regulation of cell behavior. In the present study, poly (L-glutamic acid) (PLG) hydrogels were prepared by catalyst-free click crosslinking via a strain-promoted azide-alkyne cycloaddition (SPAAC) reaction between azido-grafted PLG (PLG-N3) and azadibenzocyclooctyne-grafted PLG (PLG-ADIBO). The bioactive peptides c(RGDfK) and N-cadherin mimetic peptide (N-Cad) were both conjugated to the PLG hydrogel (denoted PLG+RGD/N-Cad) in order to regulate cell-material and cell-cell interactions. Gelation time and storage modulus of the hydrogels were tunable through variations in the concentration of polypeptide precursors. The hydrogels degraded gradually in the presence of proteinases. The viability of bone marrow mesenchymal stem cells (BMSCs) was maintained when cultured with extracts of the hydrogels or encapsulated within the hydrogels. Degradation was observed within 10 weeks following the subcutaneous injection of hydrogel solution in rats, displaying excellent histocompatibility in vivo. The introduction of RGD into the PLG hydrogel promoted the adhesion of BMSCs onto the hydrogels. Moreover, when encapsulated within the PLG+RGD/N-Cad hydrogel, BMSCs secreted cartilage-specific matrix, in addition to chondrogenic gene and protein expression being significantly enhanced in comparison with BMSCs encapsulated in hydrogels without N-Cad modification. These findings suggest that these biodegradable, bioactive polypeptide hydrogels have great potential for use in 3D cell culture and in cartilage tissue engineering.



中文翻译:

RGD和N-钙粘蛋白模拟肽修饰的生物活性多肽水凝胶可促进骨髓间充质干细胞的软骨分化

细胞材料和细胞间的相互作用代表了调节细胞行为的两个关键方面。在本研究中,通过叠氮接枝的PLG(PLG-N 3)之间的应变促进的叠氮化物-炔烃环加成(SPAAC)反应,通过无催化剂的点击交联制备了聚(L-谷氨酸)(PLG)水凝胶。)和氮杂二苯并环辛炔接枝的PLG(PLG-ADIBO)。将生物活性肽c(RGDfK)和N-钙粘蛋白模拟肽(N-Cad)都缀合到PLG水凝胶(表示为PLG + RGD / N-Cad),以调节细胞材料和细胞之间的相互作用。水凝胶的胶凝时间和储能模量可通过改变多肽前体的浓度来调节。在蛋白酶存在下,水凝胶逐渐降解。用水凝胶提取物或包裹在水凝胶中培养时,骨髓间充质干细胞(BMSC)的活力得以维持。皮下注射大鼠水凝胶溶液后10周内观察到降解,显示出优异的体内组织相容性。将RGD引入PLG水凝胶中促进了BMSCs在水凝胶上的粘附。此外,与包封在未经N-Cad修饰的水凝胶中的BMSC相比,BMSCs在囊封在PLG + RGD / N-Cad水凝胶中时,除了会显着增强软骨生成基因和蛋白质表达外,还分泌软骨特异性基质。这些发现表明,这些可生物降解的生物活性多肽水凝胶在3D细胞培养和软骨组织工程中具有巨大的潜力。

更新日期:2020-06-08
down
wechat
bug