Due to its high commercial value, many studies on rose (Rosa hybrida L.) micropropagation have been published. However, there are a limited number of studies on rose in vitro flowering. These studies only focused on the roles of plant growth regulators in the formation and morphogenesis of flowers. In this protocol, cytokinin was confirmed to positively function in the induction of in vitro rose flowers. In fact, more than 40% of in vitro shoots were induced to flower when they were grown on a medium supplemented with benzylaminopurine (BA) (2 mg L⁻¹) and IAA (0.1 mg L⁻¹). In addition, this study showed that the growth medium supplemented with only coconut water (15 or 20% v/v) was very efficient to induce flowering of in vitro miniature rose plants (> 70%) after 60 d of subculture. In addition, the in vitro flowers were normal and almost similar to ex vitro flowers in terms of flower shape and color. Based on these results, a detailed procedure for in vitro miniature rose flower production is provided.

由于其高商业价值，已经发表了许多关于玫瑰（Rosa hybrida L.）微繁殖的研究。但是，关于玫瑰体外开花的研究很少。这些研究仅集中在植物生长调节剂在花的形成和形态发生中的作用。在该协议中，确认细胞分裂素在体外玫瑰花的诱导中起正作用。实际上，当它们在补充有苄基氨基嘌呤（BA）（2 mg L ^-1）和IAA（0.1 mg L ^-1）的培养基上生长时，超过40％的体外芽被诱导开花。此外，这项研究表明，在生长培养基中仅添加椰子水（15％或20％v / v）在传代培养60天后非常有效地诱导了体外微型玫瑰植物（> 70％）的开花。另外，离体花是正常的，并且在花的形状和颜色方面几乎与离体花相似。基于这些结果，提供了用于体外微型玫瑰花生产的详细程序。