In order to induce in vitro axillary shoot proliferation from single-node explants of Rhododendron mucronulatum Turcz., two techniques of thidiazuron (TDZ) application were tested: (i) two-step procedure including cultivation on Anderson medium (AM) supplemented with varying TDZ concentrations (0.1 μM; 0.25 μM; 0.5 μM; 1.0 μM; 2.5 μM) for 8 wk followed by cultivation on hormone-free medium (AM0) for 6 wk and (ii) 4-h liquid-pulse treatment with different TDZ concentrations (7.5 μM, 15.0 μM, or 30.0 μM) followed by cultivation on AM0 for 8 wk. The highest number of axillary shoots per explant was achieved with 0.1-μM TDZ after the two-step procedure. The best response in terms of percent regeneration (87%), shoot length (13 mm), absence of structure anomalies, and the shortest shoot production cycle (8 wk) was obtained with 30.0-μM TDZ liquid-pulse treatment for 4 h. The clonal fidelity of regenerated shoots was evaluated by random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers and flow cytometry. Genetic similarity of all regenerants between themselves and with the mother seedlings was 99%. Flow cytometric analysis revealed that all samples studied were diploid. The nuclear DNA content of microshoots obtained under the TDZ treatments varied from 1.26 to 1.32 pg per 2C. There were no significant differences in DNA content among mother seedlings and in vitro developed shoots triggered by 0.1- and 2.5-μM TDZ nor by those triggered by the 30.0-μM TDZ pulse treatment.

为了诱导体外从单节外植腋生芽增殖迎红杜鹃Turcz。，测试了噻唑隆（TDZ）的两种应用技术：（i）两步程序，包括在补充了不同TDZ浓度（0.1μM; 0.25μM; 0.5μM; 1.0μM; 2.5μM）的安德森培养基（AM）上培养8周，然后在无激素培养基（AM0）上培养6周，以及（ii）用不同TDZ浓度（7.5μM，15.0μM或30.0μM）进行4小时液体脉冲处理，然后在AM0上培养8周周。经过两步操作后，使用0.1μMTDZ可以使每个外植体的腋芽数量最多。用30.0-μMTDZ液体脉冲处理4 h获得了最佳的响应，包括再生百分数（87％），枝条长度（13 mm），无结构异常和最短的枝条生产周期（8 wk）。通过随机扩增的多态性DNA（RAPD）和简单序列间重复（ISSR）标记以及流式细胞仪评估再生枝的克隆保真度。自身与母本之间所有再生子的遗传相似性均为99％。流式细胞仪分析显示，所有研究样品均为二倍体。在TDZ处理下获得的微芽的核DNA含量从每2C 1.26 pg至1.32 pg不等。母本和母本间DNA含量没有显着差异。每2C为26至1.32 pg。母本和母本间DNA含量没有显着差异。每2C为26至1.32 pg。母本和母本间DNA含量没有显着差异。体外发育的芽由0.1和2.5μMTDZ触发，也不受30.0μMTDZ脉冲处理触发。