Chestnut species have large ecological, cultural and economic importance. Developing genetic markers for these species is of interest for conservation, breeding or evolutionary studies. We designed 192 primer pairs targeting microsatellites detected in the Castanea mollissima reference genome and tested them on C. sativa and C. crenata. We PCR amplified 3 × 50 microsatellites in 106 chestnut trees. Microhaplotype calling accounting for all polymorphisms resulted in a total of 98 high confidence polymorphic markers. Mean number of haplotypes per marker was 9.05 with respectively 71%, 12% and 16% of the variation corresponding to microsatellite variation in repeats number, SNP within the repeat motif and SNP or INDEL in the flanking sequence. Overall, the simple protocol described here generated a powerful multilocus genetic dataset for chestnut genetic investigations.

栗树具有重要的生态，文化和经济意义。为这些物种开发遗传标记对于保护，育种或进化研究很重要。我们设计了192种针对在板栗参照基因组中检测到的微卫星的引物对，并在C. sativa和C. crenata上进行了测试。。我们在106棵栗树中PCR扩增了3×50个微卫星。要求所有多态性的微单倍型导致总共98个高置信度多态性标记。每个标记的单倍型平均数为9.05，分别有71％，12％和16％的变异与重复序列的微卫星变异，重复基序内的SNP和侧翼序列中的SNP或INDEL相对应。总的来说，这里描述的简单协议为栗子遗传研究生成了一个强大的多基因座遗传数据集。