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Genetic diversity, antimicrobial resistance, and virulence genes of thermophilic Campylobacter isolated from broiler production chain
Brazilian Journal of Microbiology ( IF 2.2 ) Pub Date : 2020-06-09 , DOI: 10.1007/s42770-020-00314-0
Tassiana Ramires 1 , Mauricéia Greici de Oliveira 1 , Natalie Rauber Kleinubing 1 , Simone de Fátima Rauber Würfel 2 , Marcia Magalhães Mata 1 , Mariana Almeida Iglesias 2 , Graciela Volz Lopes 1 , Odir Antônio Dellagostin 2 , Wladimir Padilha da Silva 1, 2
Affiliation  

The aim of this study was to investigate the prevalence of thermophilic Campylobacter in the broiler production chain of southern Brazil, by evaluating broiler farms and slaughter line samples, and to determine the genetic diversity, antimicrobial resistance, and virulence genes of the isolates. Of the 140 samples investigated in this study, 75 (53.6%) were positive for thermophilic Campylobacter, and all isolates were identified by phenotypic and molecular tests as C. jejuni. The resistance to nalidixic acid was the most common (74%), followed by resistance to enrofloxacin (67.3%) and ciprofloxacin (37.1%). However, there was no resistance to the macrolides tested which are recommended for the treatment of human campylobacteriosis. The PFGE showed that the isolates were grouped in eight macrorestriction patterns (P1 to P8). A representative isolate of each macrorestriction pattern was investigated for the presence of virulence genes and all isolates carried the cadF, ciaB, cdtA, cdtB, cdtC, and flaA genes. The dnaJ gene was detected in 87.5% (7/8) of the isolates. The flhA and racR genes were detected in 75% (6/8), while the pldA gene was present in 62.5% (5/8) and the wlaN gene in 25% (2/8). The presence of C. jejuni in broiler farms and in the slaughterhouse is a hazard to consumer given that this pathogen can be maintained throughout the broiler production chain and contaminates the final product. Moreover, the presence of the major virulence genes in the isolates demonstrates that they have the ability to develop campylobacteriosis in humans.

中文翻译:

肉鸡生产链中分离的嗜热弯曲杆菌的遗传多样性、抗菌素耐药性和毒力基因

本研究的目的是通过评估肉鸡养殖场和屠宰线样本,调查巴西南部肉鸡生产链中嗜热弯曲杆菌的流行情况,并确定分离株的遗传多样性、抗菌素耐药性和毒力基因。在本研究中调查的 140 个样本中,75 个 (53.6%) 嗜热弯曲杆菌呈阳性,所有分离株均通过表型和分子测试鉴定为空肠弯曲杆菌。萘啶酸耐药最常见(74%),其次是恩诺沙星(67.3%)和环丙沙星(37.1%)。然而,对推荐用于治疗人类弯曲杆菌病的大环内​​酯类没有抗性。PFGE 显示分离株分为八种宏观限制模式(P1 到 P8)。研究了每个宏观限制模式的代表性分离株是否存在毒力基因,所有分离株都携带 cadF、ciaB、cdtA、cdtB、cdtC 和 flaA 基因。在 87.5% (7/8) 的分离株中检测到 dnaJ 基因。flhA 和 racR 基因的检测率为 75% (6/8),而 pldA 基因的检测率为 62.5% (5/8),而 wlaN 基因的检测率为 25% (2/8)。肉鸡养殖场和屠宰场中空肠弯曲杆菌的存在对消费者构成危害,因为这种病原体可以在整个肉鸡生产链中保持并污染最终产品。此外,分离株中主要毒力基因的存在表明它们具有在人类中发展弯曲杆菌病的能力。ciaB、cdtA、cdtB、cdtC 和 flaA 基因。在 87.5% (7/8) 的分离株中检测到 dnaJ 基因。75% (6/8) 检测到 flhA 和 racR 基因,62.5% (5/8) 检测到 pldA 基因,25% (2/8) 检测到 wlaN 基因。肉鸡养殖场和屠宰场中空肠弯曲杆菌的存在对消费者构成危害,因为这种病原体可以在整个肉鸡生产链中保持并污染最终产品。此外,分离株中主要毒力基因的存在表明它们具有在人类中发展弯曲杆菌病的能力。ciaB、cdtA、cdtB、cdtC 和 flaA 基因。在 87.5% (7/8) 的分离株中检测到 dnaJ 基因。flhA 和 racR 基因的检测率为 75% (6/8),而 pldA 基因的检测率为 62.5% (5/8),而 wlaN 基因的检测率为 25% (2/8)。肉鸡养殖场和屠宰场中空肠弯曲杆菌的存在对消费者构成危害,因为这种病原体可以在整个肉鸡生产链中保持并污染最终产品。此外,分离株中主要毒力基因的存在表明它们具有在人类中发展弯曲杆菌病的能力。肉鸡养殖场和屠宰场中的空肠菌对消费者构成危害,因为这种病原体可以在整个肉鸡生产链中保持并污染最终产品。此外,分离株中主要毒力基因的存在表明它们具有在人类中发展弯曲杆菌病的能力。肉鸡养殖场和屠宰场中的空肠菌对消费者构成危害,因为这种病原体可以在整个肉鸡生产链中保持并污染最终产品。此外,分离株中主要毒力基因的存在表明它们具有在人类中发展弯曲杆菌病的能力。
更新日期:2020-06-09
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