Aim and Objective: Oxidative stress is implicated in the development and progression of many diseases. Some of the appropriate actions that could taken to resolve the problem of these diseases are search for new antioxidant substances isolated from plants. The aims of this study were to research the intraspecies variations of A. verticillata and C. caeruleus essential oils from 8 locations using statistical analysis, the in vitro antioxidant properties of collective essential oils and in combinations.

Materials and Methods: The essential oils were analyzed by GC and GC-MS. The intraspecies variations of the essential oil compositions were discussed using principal component analysis (PCA) and cluster analysis (CA). The antioxidant properties were evaluated DPPH-radical scavenging activity and β-carotene bleaching test.

Results: The main components of Ammoides verticillata collective essential oil (Coll EO) were thymol (30.5%), carvacrol (23.2%), p-cymene (13.1%), limonene (12.5%) and terpinene-4-ol (12.3%). While roots of Carthamus caeruleus essential oil were dominated by carline oxide (86.2%). The chemical variability allowed the discrimination of two main Groups for both Coll EOs. A direct correlation between the altitudes, climate and the chemical compositions was evidenced. Ammoides verticulata and Carthamus caeruleus Coll Eos showed good antioxidant activity. In binary mixture, the interaction between both Coll Eos and between oils rich of thymol and/or carvacrol with carlina oxide produced the best synergistic effects compared to individual essential oils and the synthetic antioxidant (BHT).

Conclusion: Ammoides verticillata and Carthamus caeruleus essential oil blends can be used as a natural food preservative and alternative to chemical antioxidants.

目的与目的：氧化应激与多种疾病的发生发展有关。一些可以用来解决这些疾病问题的适当行动是寻找从植物中分离出来的新的抗氧化物质。本研究的目的是使用统计分析、集体精油及其组合的体外抗氧化特性研究来自 8 个地点的轮枝花和蓝蓝花精油的种内变异。

材料和方法：通过GC和GC-MS分析精油。使用主成分分析 (PCA) 和聚类分析 (CA) 讨论了精油成分的种内变化。抗氧化性能通过DPPH-自由基清除活性和β-胡萝卜素漂白试验进行评价。

结果：轮枝菊集体精油（Col EO）的主要成分为百里酚（30.5%）、香芹酚（23.2%）、对伞花烃（13.1%）、柠檬烯（12.5%）和萜品烯-4-醇（12.3%） ）。而Carthamus caeruleus精油的根以氧化carline (86.2%)为主。化学变异性允许区分两个 Coll EO 的两个主要组。高度、气候和化学成分之间的直接相关性得到证实。Ammoides verticulata 和 Carthamus caeruleus Coll Eos 显示出良好的抗氧化活性。在二元混合物中，与单独的精油和合成抗氧化剂 (BHT) 相比，Coll Eos 之间以及富含百里酚和/或香芹酚的油与氧化 carlina 之间的相互作用产生了最佳的协同效应。

结论：轮枝菊和红花精油混合可用作天然食品防腐剂和化学抗氧化剂的替代品。