当前位置: X-MOL 学术Curr. Microbiol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Luteimonas granuli sp. nov., Isolated from Granules of the Wastewater Treatment Plant
Current Microbiology ( IF 2.6 ) Pub Date : 2020-06-05 , DOI: 10.1007/s00284-020-02066-4
Muhammad Zubair Siddiqi 1, 2 , Jun Mo Yeon 3 , Hanna Choi 3 , Jae Hag Lee 4 , Sang Yong Kim 5 , Ji-Hyang Wee 5 , Wan Taek Im 1, 2
Affiliation  

A Gram-reaction negative, aerobic, non-motile, light yellow colored, and rod-shaped bacterium (designated Gr-4T) isolated from granules of a wastewater treatment plant, was characterized by a polyphasic approach to clarify its taxonomic position. Strain Gr-4T was observed to grew optimally at 30 ºC and at pH 7.0 on R2A medium. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Gr-4T belongs to the genus Luteimonas of the family Xanthomonadaceae and was most closely related to Luteimonas padinae CDR SL 15T (99.1%), Luteimonas terricola DSM 22344T (98.5%) and Luteimonas arsenica 26-35T (97.6). The genome comprises 2,917,404 bp with a G+C content of 70.5 mol%. The ANI value between strain Gr-4T and Luteimonas padinae CDR SL 15T was 87.3%. The DNA–DNA relatedness value between strain Gr-4T and Luteimonas padinae CDR SL 15T, Luteimonas terricola DSM 22344T was 36.4 ± 1.3% and 14.2 ± 1.7%, respectively. The predominant quinone was Q-8. The major fatty acids were iso-C15:0, iso-C16:0 and summed feature 9 (comprising iso-C17:1ω9c and/or C16:0 10-methyl) supported the affiliation of strain Gr-4T to the genus Luteimonas. Moreover, the physiological, biochemical results, and low level of ANI and DNA–DNA relatedness value allowed the phenotypic and genotypic differentiation of strains Gr-4T from other Luteimonas species with validly published names. The novel isolate therefore represents a novel species, for which the name Luteimonas granuli sp. nov. is proposed, with the type strain Gr-4T (=KACC 16614T = JCM 18203T).

中文翻译:

Luteimonas granuli sp。十一月,从污水处理厂的颗粒中分离出来

从废水处理厂的颗粒中分离出的革兰氏反应阴性、需氧、非运动、浅黄色和杆状细菌(指定为 Gr-4T),通过多相方法来阐明其分类位置。观察到菌株 Gr-4T 在 30 ºC 和 pH 7.0 下在 R2A 培养基上最佳生长。基于 16S rRNA 基因序列的系统发育分析表明,菌株 Gr-4T 属于黄单胞菌科黄单胞菌属,与 Luteimonas padinae CDR SL 15T (99.1%)、Luteimonas terricola DSM 22344T 和 Luteimonas terricola DSM 22344T (98.5%) 的亲缘关系最密切。 26-35T (97.6)。基因组包含 2,917,404 bp,G+C 含量为 70.5 mol%。菌株 Gr-4T 和 Luteimonas padinae CDR SL 15T 之间的 ANI 值为 87.3%。菌株 Gr-4T 和 Luteimonas padinae CDR SL 15T 之间的 DNA-DNA 相关性值,Luteimonas terricola DSM 22344T 分别为 36.4 ± 1.3% 和 14.2 ± 1.7%。主要的醌是Q-8。主要脂肪酸是 iso-C15:0、iso-C16:0 和总特征 9(包括 iso-C17:1ω9c 和/或 C16:0 10-甲基)支持菌株 Gr-4T 与黄褐藻属的隶属关系。此外,生理、生化结果以及 ANI 和 DNA-DNA 相关性值的低水平允许 Gr-4T 菌株与具有有效公布名称的其他 Luteimonas 物种的表型和基因型分化。因此,新的分离株代表了一种新的物种,其名称为 Luteimonas granuli sp。十一月 提出,类型应变 Gr-4T (=KACC 16614T = JCM 18203T)。0 和总特征 9(包含异 C17:1ω9c 和/或 C16:0 10-甲基)支持菌株 Gr-4T 与黄褐藻属的隶属关系。此外,生理、生化结果以及 ANI 和 DNA-DNA 相关性值的低水平允许 Gr-4T 菌株与具有有效公布名称的其他 Luteimonas 物种的表型和基因型分化。因此,新的分离株代表了一种新的物种,其名称为 Luteimonas granuli sp。十一月 提出,类型应变 Gr-4T (=KACC 16614T = JCM 18203T)。0 和总特征 9(包含异 C17:1ω9c 和/或 C16:0 10-甲基)支持菌株 Gr-4T 与黄褐藻属的隶属关系。此外,生理、生化结果以及 ANI 和 DNA-DNA 相关性值的低水平允许 Gr-4T 菌株与具有有效公布名称的其他 Luteimonas 物种的表型和基因型分化。因此,新的分离株代表了一种新的物种,其名称为 Luteimonas granuli sp。十一月 提出,类型应变 Gr-4T (=KACC 16614T = JCM 18203T)。低水平的 ANI 和 DNA-DNA 相关性值允许 Gr-4T 菌株与具有有效公布名称的其他 Luteimonas 物种的表型和基因型分化。因此,新的分离株代表了一种新的物种,其名称为 Luteimonas granuli sp。十一月 提出,类型应变 Gr-4T (=KACC 16614T = JCM 18203T)。低水平的 ANI 和 DNA-DNA 相关性值允许 Gr-4T 菌株与具有有效公布名称的其他 Luteimonas 物种的表型和基因型分化。因此,新的分离株代表了一种新的物种,其名称为 Luteimonas granuli sp。十一月 提出,类型应变 Gr-4T (=KACC 16614T = JCM 18203T)。
更新日期:2020-06-05
down
wechat
bug