Background: Intracanal disinfection is a critical, yet challenging goal for long-term success in regenerative-based treatments. This in-vitro study aimed to assess the release profile of triple antibiotic- eluting Injectable Platelet-Rich Fibrin (I-PRF) constructs in 28 days.

Methods: I-PRF scaffolds containing triple antibiotic mixture [Metronidazole (MET), Ciprofloxacin (CIP), and Minocycline (MINO)] by immersion (group one), I-PRF scaffolds containing triple antibiotic mixture by integration (group two), and antibiotic-free I-PRF scaffolds (group three) were fabricated. The antibiotic release from the scaffolds was measured using High-Performance Liquid Chromatography (HPLC) (the mobile phase of 0.1% formic acid and methanol (35:65 v/v), a C18 analytical column (150 × 4.6 mm, 5 μm) at a flow rate of 0.7 mL/min, at 25ºC) at days 1, 3, 7, 14, 21, and 28.

Results: Retention times for MINO, CIP, and MET were achieved as 2.3, 2.6, and 3.1 min, respectively. The maximum UV absorbance values for CIP, MET, and MINO were 268 nm, 278 nm, and 350 nm, respectively. The results of the first group showed burst release within the first 24 hours followed by sustained maintenance of all three antibiotics up to 14 days. MINO and MET were still detectable in the third week. The second group could not sustainably release the antibiotics.

Conclusion: The developed method for the simultaneous identification and quantification of each antibiotic in I-PRF was sensitive and quick. Overall, group one could take up the antibiotics in adequate quantities and then subsequently release them over the study period.

背景：根管内消毒是基于再生治疗的长期成功的关键但具有挑战性的目标。这项体外研究旨在评估三联抗生素洗脱的可注射富含血小板的纤维蛋白（I-PRF）构建物在28天内的释放曲线。

方法：通过浸入将三联抗生素混合物[甲硝唑（MET），环丙沙星（CIP）和米诺环素（MINO）]混合在一起的I-PRF支架（第一组），将三联抗生素混合物进行整合的I-PRF支架（第二组），以及制作了无抗生素的I-PRF支架（第三组）。使用高效液相色谱（HPLC）（0.1％甲酸和甲醇（35:65 v / v）的流动相），C18分析柱（150×4.6 mm，5μm）测量从支架释放的抗生素第1、3、7、14、21和28天的流量为0.7 mL / min（在25ºC下）。

结果：MINO，CIP和MET的保留时间分别为2.3、2.6和3.1分钟。CIP，MET和MINO的最大UV吸收值分别为268 nm，278 nm和350 nm。第一组的结果显示在最初的24小时内爆发释放，然后持续维持所有三种抗生素直到14天。第三周仍可检测到MINO和MET。第二组不能持续释放抗生素。

结论：建立的同时鉴定和定量I-PRF中每种抗生素的方法灵敏，快速。总体而言，第一组可以吸收足够量的抗生素，然后在研究期内将其释放。