RNA sequencing and DNA methylomic profiling were performed after differentiating monocytes for 6 days into moDCs with/without CXCL4 presence. We show that CXCL4 downregulates genes associated with tolerogenicity in DCs including C1Q. Expression profiles of C1Q genes were negatively correlated with their DNA methylation profiles and with immunogenic genes.

中文翻译：

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CXCL4抑制单核细胞衍生的树突状细胞的耐受性免疫特征。

在将单核细胞分化为有/无CXCL4的moDC中连续6天后，进行RNA测序和DNA甲基化分析。我们表明，CXCL4下调与DCs包括C1Q的致耐受性相关的基因。C1Q基因的表达谱与它们的DNA甲基化谱和免疫原性基因呈负相关。