As sessile organisms, plants must properly coordinate their growth and developmental programs with changes in the environment. The integration of exogenous environmental cues with endogenous plant hormone responses often occurs through physical protein–protein interactions (PPIs). However, a comprehensive PPI network that mediates environmental and hormonal responses has not been established. In this study, we initially cloned 113 phytohormone‐related genes and 29 light signaling components of Arabidopsis and then individually tested their mutual interactions (in total 2,655 tests) using a yeast‐two‐hybrid approach to ultimately identify 141 interactions. Based on these interaction results, we next revealed the signaling cross talk between jasmonate and abscisic acid by characterizing the JAZ1‐PYL4 and JAZ1‐ABI1 interactions. Thus, we generated a useful resource for the community to explore the molecular mechanisms underlying signaling interactions between plant hormones and/or with light.

中文翻译：

---

点对点蛋白质-蛋白质相互作用测定揭示了植物激素和环境线索之间的信号相互作用。

作为固着生物，植物必须适当地协调其生长和发育程序与环境的变化。外源环境因素与内源植物激素反应的整合通常通过物理蛋白质-蛋白质相互作用（PPI）发生。然而，介导环境和激素反应的综合 PPI 网络尚未建立。在这项研究中，我们最初克隆了拟南芥的 113 个植物激素相关基因和 29 个光信号成分，然后使用酵母双杂交方法单独测试了它们的相互相互作用（总共 2,655 次测试），最终确定了 141 种相互作用。基于这些相互作用结果，我们接下来通过表征 JAZ1-PYL4 和 JAZ1-ABI1 相互作用揭示了茉莉酸和脱落酸之间的信号串扰。因此，我们为社区探索植物激素和/或光之间信号相互作用的分子机制提供了有用的资源。