Introduction

The objective of this study was to evaluate the cell viability of layered cell sheets, irradiated with 222 nm UV light.

Methods

UV transmittance of 222 nm and 254 nm was evaluated when the cell sheets of NCTC Clone 929 cells were irradiated UV light. Cell viability was evaluated after irradiation of 222 nm using 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay. Following irradiation of two layered cell sheets at 500 mJ/cm², the cell damage of lower layers was evaluated by a colony formation and MTT assays.

Results

The UV transmittance of 222 nm was 10 times less than that of 254 nm. A MTT assay revealed that cells of cell sheets irradiated at 222 nm was less damaged than those at 254 nm, when irradiated at 5 mJ/cm². Cell colonies were formed for cells of lower layers irradiated at 222 nm whereas no colony formation was observed for those irradiated at 254 nm. Significantly higher MTT activity was observed for cells of lower layers irradiated at 222 nm than at 254 nm.

Conclusions

It is concluded that 222 nm irradiation is biologically safe for cell viability.

中文翻译：

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222 nm 紫外光照射后层状细胞片的活力评估。

介绍

本研究的目的是评估层状细胞片的细胞活力，用 222 nm 紫外光照射。

方法

当 NCTC Clone 929 细胞的细胞片被紫外线照射时，评估了 222 nm 和 254 nm 的紫外线透射率。使用 3-(4,5-二甲基-2-噻唑基)-2,5-二苯基溴化四唑 (MTT) 测定法在 222 nm 照射后评估细胞活力。^{在以 500 mJ/cm 2}照射两层细胞片后，通过集落形成和 MTT 测定评估下层的细胞损伤。

结果

222 nm 的紫外透过率比 254 nm 低 10 倍。^{MTT 分析表明，当以 5 mJ/cm 2}照射时，在 222 nm 照射下的细胞片细胞比在 254 nm 照射下的细胞损伤更小。下层细胞在 222 nm 照射下形成细胞集落，而在 254 nm 照射下未观察到集落形成。在 222 nm 下照射的下层细胞的 MTT 活性显着高于在 254 nm 下照射的细胞。

结论

得出的结论是，222 nm 照射对于细胞活力在生物学上是安全的。