On-site multiplex biosensors for innate immunity antibodies are ideal tools for monitoring health status of individuals against various diseases. This study introduces a novel antibody immunoassay testing platform incorporating microfiber-based arrays of antigens to capture specific antibodies. The fabrication and setup of the device revolved around electrospun polystyrene (ESPS) microfibers that act as three-dimensional membrane filters, capable of rapid and multifold analyte capture. In particular, the ESPS microfibers were patterned through localized oxygen plasma to create hydrophilic zones that facilitate fluid flows and immobilizations of antigens. The bulk of this robust antibody immunoassay platform could be installed into a compact syringe-driven cassette device, which could perform multiplex antibody immunoassay for antibodies specifically against Middle East respiratory syndrome coronavirus (MERS-CoV) with rapid preparation amounting to a total of 5 min, as well as high sensitivity and specificity for the MERS-CoV down to 200 μg/mL.

中文翻译：

---

基于多重微纤维的快速免疫分析，用于抗MERS-CoV抗体检测。

用于先天免疫抗体的现场多重生物传感器是监测个人针对各种疾病的健康状况的理想工具。这项研究引入了一种新型抗体免疫测定测试平台，该平台结合了基于微纤维的抗原阵列来捕获特异性抗体。该设备的制造和设置围绕着电纺聚苯乙烯（ESPS）超细纤维展开，该超细纤维充当三维膜过滤器，能够快速，多次捕获分析物。特别是，通过局部氧等离子体对ESPS微纤维进行构图，以形成有助于流体流动和抗原固定的亲水区。可以将这种强大的抗体免疫测定平台的大部分安装到紧凑型注射器驱动的盒式设备中，