Onions can be damaged by Fusarium basal rot caused by the soilborne fungus Fusarium oxysporum f. sp. cepae (FOC). Control of this pathogen is challenging since there is limited genetic resistance in onion. The identification of molecules that inhibit this pathogen is needed. Antagonism screening showed Brevibacillus fortis NRS-1210 secreted antifungal compounds into growth medium. The spent growth medium, diluted 1:1, inhibited growth of FOC conidia after seven hours and killed 67-91% of conidia after 11 h. The spent medium also inhibited growth of propagules from F. graminearum, F. proliferatum, F. verticillioides and Galactomyces citri-aurantii. Full strength spent growth medium did not effectively kill FOC conidia and chlamydospores inoculated into a sand cornmeal mixture. In silico analysis of the B. fortis NRS-1210 genome indicated the biosynthetic clusters of several antibiotics. Fractionation of spent medium followed by reverse-phase liquid chromatography with tandem mass spectrometry analysis found that fractions with the most antifungal activity contained a combination of edeines A, B and F and no other recognized antibiotics. 1H NMR signals of the active fraction corresponded to edeine, a pentapeptide with broad spectrum antimicrobial activity which blocks translation in both prokaryotes and eukaryotes. Comparative genomics of Brevibacillus genomes shows edeine producers form a clade which consists of: Brevibacillus brevis, Brevibacillus formosus, 'Brevibacillus antibioticus', Brevibacillus schisleri, Brevibacillus fortis, and Brevibacillus porteri. This observation suggests edeine played an important role in the evolution and speciation of the Brevibacillus genus.

中文翻译：

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Fortis短杆菌NRS-1210产生的乙二胺抑制洋葱病原体镰刀菌f的分生孢子和衣原体的体外生长。sp。cepae。

土生真菌Fusarium oxysporum f引起的镰刀菌基腐会损坏洋葱。sp。头皮（FOC）。由于洋葱的遗传抗性有限，因此控制这种病原体具有挑战性。需要鉴定抑制这种病原体的分子。拮抗作用筛选显示富通短杆菌NRS-1210将抗真菌化合物分泌到生长培养基中。用过的生长培养基（1：1稀释）在7小时后抑制FOC分生孢子的生长，并在11 h后杀死67-91％的分生孢子。用过的培养基还抑制了来自禾谷镰刀菌，繁殖镰刀菌，网状镰刀菌和柠檬半乳杆菌的繁殖体的生长。完全强度的用过的生长培养基不能有效杀死接种在砂玉米粉混合物中的FOC分生孢子和衣原体孢子。B的计算机分析。富通NRS-1210基因组表明了几种抗生素的生物合成簇。分馏用过的培养基，然后进行反相液相色谱和串联质谱分析，发现具有最大抗真菌活性的馏分包含乙胺乙，乙和乙的组合，不含其他公认的抗生素。活性级分的1 H NMR信号对应于edeine，这是一种具有广谱抗菌活性的五肽，可阻断原核生物和真核生物的翻译。短杆菌基因组的比较基因组学表明，乙二胺生产者形成了进化枝，其由以下组成：短短杆菌，福氏短杆菌，抗生素短杆菌，裂殖短杆菌，富通短杆菌和Porteri短杆菌。