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Biosynthesis of antioxidative enzymes and polyphenolics content in calli cultures of Prunella vulgaris L. in response to auxins and cytokinins.
Artificial Cells, Nanomedicine, and Biotechnology ( IF 5.8 ) Pub Date : 2020-06-04 , DOI: 10.1080/21691401.2020.1771349
Hina Fazal 1 , Bilal Haider Abbasi 2 , Nisar Ahmad 3 , Bushra Noureen 2 , Jahangir Shah 1 , Dan Ma 4 , Liu Chuanliang 4, 5 , Fazal Akbar 3 , Muhammad Nazir Uddin 3 , Haji Khan 1 , Mohammad Ali 3
Affiliation  

Prunella vulgaris L. is one of the therapeutic herbs containing various polyphenolics, which is used for multiple medicinal purposes. In this study, plant growth regulators (PGRs)-induced calli cultures from seed-derived leaf explants were exploited for the production of stress enzymes and polyphenolics. A growth curve was plotted for each PGR for 49 days period, which showed a distinct lag, log and decline phases. Here, the combination of naphthalene acetic acid (NAA) and 6-benzyleadenine (BA; 0.5 and 2.0 mg l-1) produced maximum fresh (6.32 FW-g/100 ml) and dry biomass (0.75 DW-g/100 ml) in contrast to control. The maximum synthesis of SOD (0.0154 FW-nM/min/mg) was detected on media comprising mixture of NAA and BA (1.5 mg l-1), while POD enzyme (0.366 FW-nM/min/mg) was higher at 0.5 mg l-1 NAA and 2, 4-dichlorophenoxy acetic acid. Further, NAA and BA (1.5 and 2.0 mg l-1) boosted up the synthesis of phenolics (18.83 GAE-mg/g-DW) and flavonoids content (18.05 RE-mg/g-DW) than control. Moreover, NAA of 1.0 and 2.0 mg l-1 were found supportive for maximum antioxidant activity (87.4%) and total protein (716 µg BSAE/mg-DW). This study will contribute in the development of cell culture in fermenter and synthesis of antioxidant secondary metabolites for commercial uses.

中文翻译:

响应植物生长素和细胞分裂素的夏枯草愈伤组织培养物中抗氧化酶和多酚含量的生物合成。

夏枯草(Prunella vulgaris L.)是一种含有多种多酚的治疗性草药,可用于多种医学用途。在这项研究中,利用植物生长调节剂(PGR)诱导的种子衍生外植体的愈伤组织培养物来生产胁迫酶和多酚。绘制了每个PGR在49天内的生长曲线,显示出明显的滞后,对数和下降阶段。在这里,萘乙酸(NAA)和6-苄基腺嘌呤(BA; 0.5和2.0 mg l-1)的组合产生最大的新鲜(6.32 FW-g / 100 ml)和干燥的生物质(0.75 DW-g / 100 ml)与控制相反。在包含NAA和BA混合物的培养基(1.5 mg l-1)上检测到SOD的最大合成(0.0154 FW-nM / min / mg),而POD酶(0.366 FW-nM / min / mg)更高,为0.5 mg 1-1 NAA和2,4-二氯苯氧基乙酸。进一步,NAA和BA(1.5和2.0 mg l-1)促进了酚类化合物(18.83 GAE-mg / g-DW)和类黄酮含量(18.05 RE-mg / g-DW)的合成。此外,NAA为1.0和2.0 mg l-1被发现有助于最大抗氧化活性(87.4%)和总蛋白(716 µg BSAE / mg-DW)。这项研究将有助于发酵罐细胞培养的发展和商业用途的抗氧化剂次级代谢产物的合成。
更新日期:2020-06-04
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