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3D printed oxidized alginate-gelatin bioink provides guidance for C2C12 muscle precursor cell orientation and differentiation via shear stress during bioprinting.
Biofabrication ( IF 9 ) Pub Date : 2020-07-08 , DOI: 10.1088/1758-5090/ab98e4 Thomas Distler 1 , Aditya A Solisito , Dominik Schneidereit , Oliver Friedrich , Rainer Detsch , Aldo R Boccaccini
Biofabrication ( IF 9 ) Pub Date : 2020-07-08 , DOI: 10.1088/1758-5090/ab98e4 Thomas Distler 1 , Aditya A Solisito , Dominik Schneidereit , Oliver Friedrich , Rainer Detsch , Aldo R Boccaccini
Affiliation
Biofabrication can be a tool to three-dimensionally (3D) print muscle cells embedded inside hydrogel biomaterials, ultimately aiming to mimic the complexity of the native muscle tissue and to create in-vitro muscle analogues for advanced repair therapies and drug testing. However, to 3D print muscle analogues of high cell alignment and synchronous contraction, the effect of biofabrication process parameters on myoblast growth has to be understood. A suitable biomaterial matrix is required to provide 3D printability as well as matrix degradation to create space for cell proliferation, matrix remodelling capacity, and cell differentiation. We demonstrate that by the proper selection of nozzle size and extrusion pressure, the shear stress during extrusion-bioprinting of mouse myoblast cells (C2C12) can achieve cell orientation when using oxidized alginate-gelatin (ADA-GEL) hydrogel bionk. The cells grow in the direction of printing, migrate to the hydrogel surface over time,...
中文翻译:
3D打印的氧化藻酸盐-明胶生物墨水可在生物打印过程中通过剪切应力为C2C12肌肉前体细胞定向和分化提供指导。
生物加工可以是在水凝胶生物材料中三维(3D)打印肌肉细胞的工具,最终目的是模仿天然肌肉组织的复杂性,并创建用于高级修复疗法和药物测试的体外肌肉类似物。然而,对于具有高细胞排列和同步收缩的3D打印肌肉类似物,必须了解生物制造工艺参数对成肌细胞生长的影响。需要合适的生物材料基质来提供3D可打印性以及基质降解,从而为细胞增殖,基质重塑能力和细胞分化创造空间。我们证明,通过正确选择喷嘴尺寸和挤出压力,当使用氧化藻酸盐-明胶(ADA-GEL)水凝胶bionk时,小鼠成肌细胞(C2C12)的挤压生物打印过程中的剪切应力可以实现细胞定向。细胞沿打印方向生长,随着时间的推移迁移到水凝胶表面,...
更新日期:2020-07-10
中文翻译:
3D打印的氧化藻酸盐-明胶生物墨水可在生物打印过程中通过剪切应力为C2C12肌肉前体细胞定向和分化提供指导。
生物加工可以是在水凝胶生物材料中三维(3D)打印肌肉细胞的工具,最终目的是模仿天然肌肉组织的复杂性,并创建用于高级修复疗法和药物测试的体外肌肉类似物。然而,对于具有高细胞排列和同步收缩的3D打印肌肉类似物,必须了解生物制造工艺参数对成肌细胞生长的影响。需要合适的生物材料基质来提供3D可打印性以及基质降解,从而为细胞增殖,基质重塑能力和细胞分化创造空间。我们证明,通过正确选择喷嘴尺寸和挤出压力,当使用氧化藻酸盐-明胶(ADA-GEL)水凝胶bionk时,小鼠成肌细胞(C2C12)的挤压生物打印过程中的剪切应力可以实现细胞定向。细胞沿打印方向生长,随着时间的推移迁移到水凝胶表面,...