Many different biofabrication approaches as well as a variety of bioinks have been developed by researchers working in the field of tissue engineering. A main challenge for bioinks often remains the difficulty to achieve shape fidelity after printing. In order to overcome this issue, a homogeneous pre-crosslinking technique, which is universally applicable to all alginate-based materials, was developed. In this study, the Young’s Modulus after post-crosslinking of selected hydrogels, as well as the chemical characterization of alginate in terms of M/G ratio and molecular weight, were determined. With our technique it was possible to markedly enhance the printability of a 2% (w/v) alginate solution, without using a higher polymer content, fillers or support structures. 3D porous scaffolds with a height of around 5 mm were printed. Furthermore, the rheological behavior of different pre-crosslinking degrees was studied. Shear forces on cells as well as the flow profile of the bioin...

中文翻译：

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改善藻酸盐在生物制造中的可印刷性：建立一种通用且均匀的预交联技术。

组织工程领域的研究人员已经开发出许多不同的生物制造方法以及各种生物墨水。生物油墨的主要挑战通常仍然是在印刷后难以获得形状保真度的困难。为了克服这个问题，开发了一种普遍适用于所有藻酸盐基材料的均质预交联技术。在这项研究中，确定了所选水凝胶的后交联后的杨氏模量，以及藻酸盐在M / G比和分子量方面的化学特征。利用我们的技术，可以显着提高2％（w / v）海藻酸盐溶液的可印刷性，而无需使用更高的聚合物含量，填料或载体结构。印刷了高度约5毫米的3D多孔支架。此外，研究了不同预交联度的流变行为。细胞上的剪切力以及生物素的流动曲线