Brevifoliol is an abeo-taxane isolated from the Taxus wallichiana needles; eighteen semisynthetic esters derivatives of brevifoliol were prepared by Steglich esterification and screened for their anti-tubercular potential against Mycobacterium tuberculosis H37Ra avirulent strain. The 3- [chloro (7)] and 3, 5-[dinitro (8)] benzoic acid ester derivatives were most active (MIC 25 ug/ml) against the pathogen. Further, in silico docking studies of the active derivative 7 with mycobacterium enzyme inhA (enoyl-ACP reductase) gave the LibDock score of 152.68 and binding energy of -208.62 and formed three hydrogen bonds with SER94, MET98, and SER94. Similarly, when derivative 8 docked with inhA, it gave the LibDock score of 113.55 and binding energy of -175.46 and formed a single hydrogen bond with GLN100 and Pi-interaction with PHE97. On the other hand, the known standard drug isoniazid (INH) gave the LibDock score of 61.63, binding energy of -81.25 and formed one hydrogen bond with ASP148. These molecular docking results and the way of binding pattern indicated that compounds 7 and 8 bound well within the binding pocket of inhA and showed a higher binding affinity than the known drug isoniazid. Additionally, both the derivatives (7 and 8) showed no cytotoxicity, with CC50 195.10 and 111.36, respectively towards the mouse bone marrow-derived macrophages.

Brevifoliol 是一种从红豆杉针叶中分离出来的 abeo-taxane；通过 Steglich 酯化制备了 18 种短叶醇的半合成酯衍生物，并筛选了它们对结核分枝杆菌 H37Ra 无毒菌株的抗结核潜力。3-[氯 (7)] 和 3, 5-[二硝基 (8)] 苯甲酸酯衍生物对病原体的活性最强 (MIC 25 ug/ml)。此外，活性衍生物 7 与分枝杆菌酶 inhA（烯酰 ACP 还原酶）的计算机对接研究给出了 152.68 的 LibDock 评分和-208.62 的结合能，并与 SER94、MET98 和 SER94 形成了三个氢键。类似地，当衍生物 8 与 inhA 对接时，它的 LibDock 得分为 113.55，结合能为 -175.46，并与 GLN100 形成单氢键，并与 PHE97 相互作用。另一方面，已知标准药物异烟肼 (INH) 的 LibDock 评分为 61.63，结合能为 -81.25，并与 ASP148 形成一个氢键。这些分子对接结果和结合模式表明，化合物 7 和 8 在 inhA 的结合口袋内结合良好，并显示出比已知药物异烟肼更高的结合亲和力。此外，这两种衍生物（7 和 8）均未显示出细胞毒性，CC50 分别为 195.10 和 111.36，对小鼠骨髓来源的巨噬细胞无毒性。