Clonostachys chloroleuca is a mycoparasite used for biocontrol of numerous fungal plant pathogens. Sequencing of the transcriptome of C. chloroleuca following mycoparasitization of the sclerotia of Sclerotinia sclerotiorum revealed significant upregulation of a mitogen-activated protein kinase (MAPK)-encoding gene, crmapk. Although MAPKs are known to regulate fungal growth and development, the function of crmapk in C. chloroleuca mycoparasitism is unclear. In this study, we investigated the role of crmapk in C. chloroleuca mycoparasitism through gene knockout and complementation. Deletion of crmapk had no influence on the C. chloroleuca morphological characteristics but could significantly reduce the mycoparasitic ability to sclerotia and biocontrol capacity to soybean Sclerotinia stem rot; crmapk complementation restored these abilities. Transcriptome analysis between Δcrmapk and the wild-type strain revealed numerous genes were significantly down-regulated after crmapk deletion, including cytochrome P450, transporters, and cell wall-degrading enzymes (CWDEs). Our findings indicate that crmapk influences C. chloroleuca mycoparasitism by regulation of genes controlling the activity of CWDEs or antibiotic production. This study provides a basis for further studies of the molecular mechanism of C. chloroleuca mycoparasitism.

中文翻译：

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丝裂原激活的蛋白激酶基因Crmapk参与了Clonostachys chloroleuca Mycoparasitism。

Clonostachys chloroleuca是一种真菌寄生虫，可用于多种真菌植物病原体的生物防治。菌核盘菌菌核霉菌化后，C。chloroleuca转录组的测序揭示了丝裂原活化蛋白激酶（MAPK）编码基因crmapk的显着上调。尽管已知MAPKs调节真菌的生长和发育，但不清楚crmapk在C. chloroleuca真菌副寄生虫中的功能。在这项研究中，我们通过基因敲除和互补研究了crmapk在C. chloroleuca真菌寄生中的作用。删除crmapk对C. chloroleuca形态特征没有影响，但是可以显着降低菌核的霉菌寄生能力和对大豆菌核茎腐病的生防能力。crmapk补充恢复了这些功能。Δcrmapk与野生型菌株之间的转录组分析表明，crmapk缺失后许多基因被显着下调，包括细胞色素P450，转运蛋白和细胞壁降解酶（CWDEs）。我们的发现表明，crmapk通过调节控制CWDEs活性或抗生素生产的基因来影响C. chloroleuca霉菌寄生。这项研究为进一步研究C. chloroleuca真菌寄生虫的分子机制提供了基础。通过控制控制CWDEs活性或抗生素生产的基因调节叶绿素霉菌。这项研究为进一步研究C. chloroleuca真菌寄生虫的分子机制提供了基础。通过控制控制CWDEs活性或抗生素生产的基因调节叶绿素霉菌。这项研究为进一步研究C. chloroleuca真菌寄生虫的分子机制提供了基础。