Here, we present a quantitative method for H₂ detection by gas chromatography–selected ion monitoring–mass spectrometry (GC-SIM-MS) using a single quadrupole instrument. Additionally, the developed method was applied to the detection of H₂ in human blood by GC-SIM-MS analysis using the existing ²²Ne in air as an internal standard (IS). H₂ was analyzed by GC-SIM-MS using a single quadrupole instrument with double TC-Molsieve 5A capillary columns for the separation of permanent gases. The detections of H₂ (analyte) and ²²Ne (IS) were performed at m/z 2 and 22, respectively, by GC-SIM-MS. The analyte and IS were separated using He as the carrier gas. The ratio of the peak area of H₂ to ²²Ne was employed to obtain a calibration curve for H₂ determination in the gas phase. The proposed GC-SIM-MS method exhibited high sensitivity in terms of the limits of detection (LOD) (1.7 ppm) and quantitation (LOQ) (5.8 ppm) for H₂ analysis. The developed quantitative assay of H₂ in the headspace of blood samples achieved high repeatability with a relative standard deviation (RSD) of 1.4 – 4.7%. We successfully detected and quantified H₂ in the headspaces of vacuum blood-collection tubes containing whole blood from 11 deceased individuals with several causes of death by employing the developed GC-SIM-MS method. The quantitative value of H₂ ranged from 5 to 905 ppm. The proposed GC-SIM-MS method was applicable to the quantitative assay of H₂ in biological samples without tedious pretreatment requirements.

在这里，我们提出了一种使用单四极杆仪器通过气相色谱-选择离子监测-质谱 (GC-SIM-MS) 检测H ₂的定量方法。此外，所开发的方法还应用于通过GC-SIM-MS分析检测人血液中的H ₂，使用空气中现有的²² Ne作为内标(IS)。使用具有双TC-Molsieve 5A毛细管柱的单四极杆仪器通过GC-SIM-MS分析H ₂以分离永久气体。通过GC-SIM-MS分别在m/z 2和22下进行H ₂（分析物）和²² Ne（IS）的检测。使用 He 作为载气分离分析物和内标。_{采用H 2}与²² Ne的峰面积之比来获得气相中H _{2测定的校准曲线。所提出的GC-SIM-MS 方法在H 2}分析的检测限(LOD) (1.7 ppm) 和定量限(LOQ) (5.8 ppm) 方面表现出高灵敏度。所开发的血液样本顶空H _{2定量分析实现了高重复性，相对标准偏差 (RSD) 为 1.4 – 4.7%。}我们采用开发的 GC-SIM-MS 方法成功地检测并定量了真空采血管顶部空间中的 H 2 ，该真空采血管含有来自 11 名具有多种死因的死者的全血_{。H 2}的定量值范围为5至905ppm。所提出的GC-SIM-MS方法适用于生物样品中H _{2的定量测定，无需繁琐的预处理要求。}