Human follicular fluid (HFF), which is composed by essential proteins required for the follicle development, provides an important microenvironment for oocyte maturation. Recently, overweight status has been considered as a detrimental impact factor on oocyte maturation, but whether HFF proteome could provide protein markers for assessing overweight-based oocyte maturation deficiency is still unknown. To reveal the HFF-based molecular characteristics associated with abnormal oocyte maturation, an iTRAQ-based comparative proteomic analysis was performed to investigate different HFF protein expression profiles from normal weight women and overweight status women. Two hundred HFF proteins were quantified in our data, of which 43% have not been overlapped by two previous publications. Compared with the HFF proteins of normal weight women, 22 up-regulated HFF proteins and 21 down-regulated HFF proteins were found in the overweight status women. PANTHER database showed these altered HFF proteins participated in development, metabolism, immunity, and coagulation, and STRING database demonstrated their complicated interaction networks. The confidence of proteomic outcome was verified by Western blot analysis of WAP four-disulfide core domain protein 2 (WFDC2), lactotransferrin (LTF), prostate-specific antigen (KLK3), fibronectin (FN1), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Further, ELISA assay indicated WFDC2 might be a potentially useful candidate HFF marker for the diagnosis of oocyte maturation arrest caused by overweight status. Our work provided a new complementary high-confidence HFF dataset involved in oocyte maturation, and these altered HFF proteins might have clinical relevance and diagnostic and prognostic value for abnormal oocyte maturation in overweight status women.

中文翻译：

---

人类卵泡液蛋白质组揭示了超重状态与卵母细胞成熟异常之间的关联。

人类卵泡液 (HFF) 由卵泡发育所需的必需蛋白质组成，为卵母细胞成熟提供了重要的微环境。最近，超重状态被认为是对卵母细胞成熟的不利影响因素，但 HFF 蛋白质组是否可以提供蛋白质标志物来评估基于超重的卵母细胞成熟缺陷仍然未知。为了揭示与异常卵母细胞成熟相关的基于 HFF 的分子特征，进行了基于 iTRAQ 的比较蛋白质组学分析，以研究来自正常体重女性和超重状态女性的不同 HFF 蛋白表达谱。在我们的数据中量化了 200 种 HFF 蛋白，其中 43% 没有与之前的两篇出版物重叠。与正常体重女性的 HFF 蛋白相比，在超重状态的女性中发现了 22 种上调的 HFF 蛋白和 21 种下调的 HFF 蛋白。PANTHER 数据库显示这些改变的 HFF 蛋白参与了发育、代谢、免疫和凝血，STRING 数据库显示了它们复杂的相互作用网络。通过 WAP 四二硫键核心结构域蛋白 2 (WFDC2)、乳转铁蛋白 (LTF)、前列腺特异性抗原 (KLK3)、纤连蛋白 (FN1) 和甘油醛 3-磷酸脱氢酶 (GAPDH) 的蛋白质印迹分析验证了蛋白质组结果的可信度）。此外，ELISA 测定表明 WFDC2 可能是潜在有用的候选 HFF 标志物，用于诊断由超重状态引起的卵母细胞成熟停滞。我们的工作提供了一个新的互补性高置信度 HFF 数据集，涉及卵母细胞成熟，