Whole genome sequencing promises to revolutionize our ability to link genotypic and phenotypic variation in a wide range of model and non-model species. Here we describe the isolation and characterization of a novel mycobacteriophage named BGlluviae that grows on Mycobacterium smegmatis mc2155. BGlluviae normally produces turbid plaques but a spontaneous clear plaque was also recovered. The genomic DNA from pure populations of the BGlluviae phage and the clear plaque mutant were sequenced. A single substitution, at amino acid 54 (I to T), in the immunity repressor protein resulted in a clear plaque phenotype. This substitution is predicted to be located at the subunit interaction interface of the repressor protein, and thus prevents the establishment of lysogeny.

中文翻译：

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全基因组测序可确定导致分枝杆菌噬菌体中清楚的与浑浊的噬菌斑形态相关的等位基因。

全基因组测序有望彻底改变我们连接各种模型和非模型物种中基因型和表型变异的能力。在这里，我们描述了一种新的分枝杆菌噬菌体的分离和特征，该噬菌体在耻垢分枝杆菌mc2155上生长，名为BGlluviae。BGlluviae通常会产生浑浊的斑块，但也可以回收自发的透明斑块。对来自BGlluviae噬菌体的纯种群和透明噬菌斑突变体的基因组DNA进行测序。免疫抑制蛋白中第54位氨基酸（I到T）的单取代导致了清晰的噬菌斑表型。预计该取代位于阻遏蛋白的亚基相互作用界面，因此阻止了溶源性的建立。