The aim of this study was to investigate the osteogenic potential of bone marrow-derived mesenchymal stem cells (BMSCs) seeded on novel thermosensitive in situ forming hydrogel systems comprising methoxy polyethylene glycol-polycaprolactone (MP) and RGD-conjugated MP (MP–RGD) in vitro and in vivo. Real-time polymerase chain reaction (PCR) together with immunofluorescence staining revealed the strong expression of osteogenic markers (collagen 1 and osteocalcin) of BMSCs in MP/MP–RGD samples compared to MP samples. PCR array testing also showed the upregulation of the interconnected signaling networks regulating cell proliferation and differentiation, which was further verified through the Kyoto Encyclopedia of Genes and Genomes pathway analysis. Histological findings and computed tomographic analysis demonstrated that the MP/MP–RGD hydrogel dramatically promoted new bone formation in a rabbit calvarial defect model. In conclusion, this hydrogel appears to elicit cellular behaviors desired for bone tissue regeneration.

中文翻译：

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基于MPEG-PCL-RGD和BMSC的可注射水凝胶，用于骨组织工程。

这项研究的目的是研究骨髓间充质干细胞（BMSCs）播种在新型热敏原位形成水凝胶系统的成骨潜能，该系统包括甲氧基聚乙二醇-聚己内酯（MP）和RGD-缀合的MP（MP-RGD）体外和体内。与MP样品相比，实时聚合酶链反应（PCR）和免疫荧光染色揭示了MP / MP-RGD样品中BMSC的成骨标记物（胶原1和骨钙蛋白）的强表达。PCR阵列测试还显示了调控细胞增殖和分化的相互连接的信号网络的上调，这一点已通过《京都议定书》的基因全书和基因组途径分析得到进一步证实。组织学结果和计算机断层扫描分析表明，MP / MP-RGD水凝胶可显着促进兔颅骨缺损模型中的新骨形成。总之，这种水凝胶似乎引发了骨组织再生所需的细胞行为。