The purpose of this study was to construct a glycyrrhetinic acid (GA)-mediated, breakable, intracellular, nanoscale drug-delivery carrier via amide and esterification reactions. The structures were identified by Fourier-transformed infrared (FTIR) and 1H-nuclear magnetic resonance (1H-NMR) spectrophotometry. The compatibility and safety of the carrier were evaluated using hemolysis and cytotoxicity tests. The GA-copolymer micelle was prepared using the solvent evaporation method. FTIR and 1H-NMR detection demonstrated the successful construction of the polymer. No hemolysis occurred in any concentration of polymer within 3 h, and the hemolysis rate was less than 5%. 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) experimental results showed that the novel polymer reduced the cell survival rate and had significant cytotoxic effects. The blank nanoparticles were liquid with light blue opalescence. Transmission electron microscopy revealed that the empty micelles were uniform spheres, with an average size of 62 nm and a zeta potential of −13 mV. The novel GA-mediated polymeric carrier material developed here has the potential to effectively kill human SMMC-7721 cancer cells within 3 days when the dose is above 500 ug/mL.

这项研究的目的是通过酰胺和酯化反应构建甘草次酸（GA）介导的，易碎的，细胞内的纳米级药物递送载体。通过傅立叶变换红外（FTIR）和1 H-核磁共振（1 H-NMR）分光光度法鉴定结构。使用溶血和细胞毒性测试评估了载体的相容性和安全性。使用溶剂蒸发法制备GA共聚物胶束。FTIR和11 H-NMR检测证明该聚合物的成功构建。任何浓度的聚合物在3 h内均未发生溶血，溶血率低于5％。3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四唑（MTT）的实验结果表明，该新型聚合物降低了细胞存活率，并具有明显的细胞毒性作用。空白的纳米颗粒为浅蓝色乳白色液体。透射电子显微镜显示，空的胶束是均匀的球体，平均大小为62 nm，ζ电位为-13 mV。当剂量超过500 ug / mL时，此处开发的新型GA介导的聚合物载体材料具有在3天内有效杀死人SMMC-7721癌细胞的潜力。