An attempt was made to study the use of bacterial species Pseudomonas putida immobilized on coconut coir pith to degrade Total Petroleum Hydrocarbon (TPH) present in soil contaminated with crude oil. The soil was spiked with crude oil to get a concentration of 10,000 mg/kg soil. The bacterial species Pseudomonas putida was immobilized on coconut coir pith and used for the degradation of crude oil. The experiment was conducted with four treatments such as T1 (Control (Soil alone)), T2 (Soil + Nutrients + Pseudomonas putida), T3 (Soil + Nutrients + Coconut coir pith), T4 (Soil + Nutrients + Pseudomonas putida immobilized in Coconut coir pith) for 60 days. During the period of the experiment the parameters viz., soil pH, temperature, moisture content, and percent degradation of the TPH compounds were determined. The results revealed that the percent degradation of the TPH was found to be highest for the treatment T4 and at 60th day maximum degradation was recorded. The percent degradation of some of the TPH compounds such as dotriacontane, octane, octacosane, octatriacontane was 45.0, 45.5, 45.2, and 44.5, respectively. The coconut coir pith immobilized with bacterial species Pseudomonas putida can, therefore, prove to be a very good biological treatment option to remediate crude oil-contaminated soils.

试图研究固定在椰壳髓上的细菌假单胞菌假单胞菌的使用，以降解存在于被原油污染的土壤中的总石油烃（TPH）。向土壤中添加原油，使其浓度为10,000 mg / kg。细菌假单胞菌恶臭假单胞菌被固定在椰壳髓上，并用于降解原油。实验采用四种处理方法进行，例如T1（对照（仅土壤），T2（土壤+营养素+假单胞菌），T3（土壤+营养素+椰子皮），T4（土壤+营养素+假单胞菌）。固定在椰子椰壳中）60天。在实验期间，确定了参数，即土壤pH，温度，水分含量和TPH化合物的降解百分比。结果表明，对于处理T4，TPH的降解百分比最高，并且在第60天记录到最大降解。某些TPH化合物（例如，六ria烷，辛烷，十八烷，八at烷）的降解百分率分别为45.0、45.5、45.2和44.5。因此，固定有细菌种恶臭假单胞菌的椰子皮可被证明是修复原油污染土壤的很好的生物处理方法。