ABSTRACT

Background: The cross-reactive allergen between mugwort (Artemisia vulgaris) and kidney bean (Phaseolus vulgaris) has not yet been identified.

Methods: A total of 24 patients were included in this study. The sera of patients were analyzed for the concentrations of specific IgE antibodies. The allergenicity and cross-reactivity were investigated by Western blotting and immunoblot inhibitory experiments.

Results: The immunoblotting indicated the binding of patients’ IgE to crude mugwort extract at ~26 kDa protein (15 cases), ~60 kDa (15 cases), and 10–15 kDa proteins (12 cases). The results of the immunoblot-inhibition assay showed that kidney bean seed extract inhibited specific IgE binding to mugwort at 10–15 kDa, ~26 kDa, and ~60 kDa in 4 (16.7%), 1 (4.2%) and 2 (8.3%) cases, respectively. On the other hand, mugwort extract was demonstrated to inhibit specific IgE binding to kidney bean seed at 10–15 kDa, 15–20 kDa, ~30 kDa, and 60 kDa in 1 (4.2%), 3 (12.5%), 4 (16.7%), and 3 (12.5%) cases, respectively.

Conclusion: The 26–30 kDa, 10–15 kDa, and 60 kDa proteins are potential causative agents of the cross-reactivity between mugwort and kidney beans. The findings of this study improved the current understanding on the allergenicity of kidney beans and would provide insights into the refinement of treatment strategy for anaphylaxis.

摘要

背景：艾蒿（Artemisia vulgaris）和芸豆（Phaseolus vulgaris）之间的交叉反应过敏原尚未确定。

方法：本研究共纳入24例患者。分析患者血清中特定 IgE 抗体的浓度。通过蛋白质印迹和免疫印迹抑制实验研究过敏性和交叉反应性。

结果：免疫印迹表明患者的 IgE 与艾蒿粗提取物的结合程度为~26 kDa 蛋白（15 例）、~60 kDa（15 例）和 10-15 kDa 蛋白（12 例）。免疫印迹抑制试验的结果表明，芸豆种子提取物在 4 (16.7%)、1 (4.2%) 和 2 (8.3 %) 例。另一方面，艾蒿提取物在 1 (4.2%)、3 (12.5%)、4 (16.7%) 和 3 (12.5%) 个案例。

结论：26-30 kDa、10-15 kDa 和 60 kDa 的蛋白质是艾蒿和芸豆之间交叉反应的潜在病原体。这项研究的结果提高了目前对芸豆过敏性的理解，并将为改进过敏反应的治疗策略提供见解。