ABSTRACT The potential of native entomopathogenic nematodes for the control of the brown marmorated stink bug Halyomorpha halys was evaluated in experimental trials in Georgia. Two Georgian strains of Heterorhabditis bacteriophora (HRB, GEO) and Steinernema borjomiense and two Italian strains of H. bacteriophora (HRB, IT) and S. apuliae were used in laboratory assays (22°C and 80% RH) at concentrations of 1000, 500 and 200 infective juveniles (IJs) per adult H. halys. The mortality of tested insects was estimated from the third day after treatment. Differences in mortality of the tested insect were observed between Georgian and Italian strains. At the highest concentration (1000 IJs/adult), mortality (%) of H. halys induced by Georgian strains HRB and S. borjomiense was 53.3 and 40, respectively, at 500 IJs/adult 40 and 33.3, and at 200 IJs/adult 33.3 and 13.3, respectively. Italian strains HRB (IT) and S. apuliae proved more suppressive against the tested insects, with mortality rates of 95.5 and 60, 93.3 and 40 and 73.3 and 33.2%, at concentrations of 1000, 500 and 200 IJs/adult of H. halys, respectively. The present study provides further insights in selection of promising EPN strains to be used against this harmful insect pest, although further studies to test their efficacy under field conditions are needed.

中文翻译：

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天然昆虫病原线虫在乔治亚州控制棕色獾臭虫 Halyomorpha halys 的潜力

摘要 在乔治亚州的实验性试验中评估了天然昆虫病原线虫控制棕色獾臭虫 Halyomorpha halys 的潜力。在实验室测定中使用了两种格鲁吉亚菌株 Heterorhabditis bacteriophora (HRB, GEO) 和 Steinernema borjomiense 以及两种意大利菌株 H. bacteriophora (HRB, IT) 和 S. apuliae，浓度为 1000，每个成年 H. halys 有 500 和 200 只感染性幼虫 (IJ)。从处理后的第三天开始估计受试昆虫的死亡率。在格鲁吉亚和意大利菌株之间观察到测试昆虫的死亡率差异。在最高浓度（1000 IJs/成人）下，由格鲁吉亚菌株 HRB 和 S. borjomiense 诱导的 H. halys 的死亡率（%）分别为 53.3 和 40，500 IJs/成人 40 和 33.3，200 IJs/成人分别为 33.3 和 13.3。意大利菌株 HRB (IT) 和 S. apuliae 被证明对受试昆虫具有更强的抑制作用，死亡率分别为 95.5%、60%、93.3% 和 40% 以及 73.3% 和 33.2%，浓度为 1000、500 和 200 IJ/H. halys 成虫， 分别。本研究为选择用于对抗这种有害昆虫的有希望的 EPN 菌株提供了进一步的见解，尽管需要进一步研究以测试它们在田间条件下的功效。