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What are the consequences of Methylphenidate exposure for maternally separated rats?
International Journal of Developmental Neuroscience ( IF 1.8 ) Pub Date : 2020-07-22 , DOI: 10.1002/jdn.10045
Fatemeh Mohtashami Borzadaran 1 , Khadijeh Esmaeilpour 1 , Sara Joushi 1 , Mohammad Amin Rajizadeh 1 , Vahid Sheibani 1 , Naeem Ur Rehman 2 , Gholamreza Sepehri 1
Affiliation  

Methylphenidate (MPH) abuse is prevalent among youth. Drug abuse results in pain perception. We sought to investigate whether Maternal separation (MS) prone to MPH addiction. The next question was whether MPH abusers with MS differ in pain perception. We investigated the impact of MS on addiction and drug reward as well as pain perception following 5 days of MPH injection in males and females rats. Initially, rats underwent MS protocol of 3 hr daily for 21 days. Conditioned place preference (CPP) test was an attempt to investigate whether MS rats experience more reward with MPH. The protocol consisted of 10 min habituation on Day 1, conditioning on Day 2–Day 6 (5 mg per kg MPH injection in drug compartment and saline in saline compartment with 4 hr gap between injections) and 10 min test on Day 7. Furthermore, using another group, differences in pain perception were investigated after 5 days of daily MPH injection with 5 mg per kg. Sensory pain sensitivity was tested on PND 39 using tail flick and hotplate in MS and control groups with and without MPH exposure. Results indicated that female rats are equally prone to addiction in CPP. On the other hand, MS males experience a higher reward in CPP. In tail flick test, female MS rats exposed to MPH show a lower sensory pain threshold with similar MPH exposure. Experiencing MPH similarly declined hotplate pain perception in MS and controls in the females. Males, on the other hand, did not show any difference in sensory pain tests. According to results one can argue MS is detrimental. MS males experience more reward with MPH, females are equally addiction prone and MS females experience more pain in tail flick. On the other hand pain threshold can decline in hotplate test for both control and MS females that received MPH.

中文翻译:

哌甲酯暴露对母体分离的大鼠有什么后果?

哌甲酯 (MPH) 滥用在青少年中很普遍。药物滥用导致疼痛感。我们试图调查母体分离 (MS) 是否容易出现 MPH 成瘾。下一个问题是患有 MS 的 MPH 滥用者的疼痛感知是否不同。我们调查了雄性和雌性大鼠 MPH 注射 5 天后 MS 对成瘾和药物奖励以及疼痛感知的影响。最初,大鼠每天接受 3 小时的 MS 方案,持续 21 天。条件性位置偏好 (CPP) 测试试图调查 MS 大鼠是否在 MPH 时体验到更多奖励。该方案包括第 1 天的 10 分钟适应、第 2 天至第 6 天的调节(在药物隔室中每公斤 MPH 注射 5 毫克,在盐水隔室中注射生理盐水,注射间隔为 4 小时)和第 7 天的 10 分钟测试。此外,使用另一个组,每天注射 5 毫克/公斤 MPH 5 天后,研究疼痛感知的差异。在有和没有 MPH 暴露的 MS 和对照组中,使用甩尾和加热板在 PND 39 上测试感官疼痛敏感性。结果表明,雌性大鼠同样容易对 CPP 上瘾。另一方面,MS 男性在 CPP 中获得更高的奖励。在甩尾试验中,暴露于 MPH 的雌性 MS 大鼠表现出较低的感觉痛阈值与类似的 MPH 暴露。经历 MPH 类似地降低了 MS 和女性对照组的热板痛觉。另一方面,男性在感官疼痛测试中没有表现出任何差异。根据结果​​,可以认为 MS 是有害的。MS 男性在 MPH 中获得更多奖励,女性同样容易上瘾,MS 女性在甩尾时经历更多痛苦。
更新日期:2020-07-22
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