当前位置：
X-MOL 学术
›
Process Biochem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Influence of ethanol concentration of extraction solvent on metabolite profiling for Salviae Miltiorrhizae Radix et Rhizoma extract by 1H NMR spectroscopy and multivariate data analysis
Process Biochemistry ( IF 4.4 ) Pub Date : 2020-10-01 , DOI: 10.1016/j.procbio.2020.06.008 Wenzhu Li , Fang Zhao , Jianyang Pan , Haibin Qu
Process Biochemistry ( IF 4.4 ) Pub Date : 2020-10-01 , DOI: 10.1016/j.procbio.2020.06.008 Wenzhu Li , Fang Zhao , Jianyang Pan , Haibin Qu
|
Abstract Salviae Miltiorrhizae Radix et Rhizoma (Danshen in China) and its related preparations are widely used in clinical practice due to its high medicinal value. In recent years, 1H NMR technology has made great progress and demonstrated its unique advantages in the field of botanical metabolomics. In this study, 1H NMR-based metabolomics was used to investigate the dissolution of various metabolites in Danshen as a function of ethanol concentration. 1H NMR spectroscopy of Danshen extract identified 28 metabolites including 6 sugars, 11 amino acids, 3 organic acids, 4 salvianolic acids, and 4 tanshinones. Multivariate statistical analysis was used to classify and compare various Danshen extracts. PCA and HCA were used to obtain a global overview of the similarity in the samples and two-class OPLS-DA models were established for identifying characteristic metabolites. Then, 1H-qNMR method was used to estimate the concentration of 22 metabolites, which is helpful to further describe the changes in metabolite ratios of various Danshen extracts. The result of this study laid the foundation for further biological activity research, and also provided an important reference for subsequent process research and quality control of Danshen related preparations.
更新日期：2020-10-01
京公网安备 11010802027423号