Abstract While methods like radiology or histology are routinely used in oral surgery, a simple and effective method for characterization of alveolar bones at the molecular level is missing. This is primarily due to the fact that proteomic analysis of hard tissue represents a serious analytical challenge. In this study, we developed a simple technique for the proteomic characterization of bone tissue in the field of oral surgery. After a prewashing step with different solvents, specific digestion with trypsin was performed directly in porcine alveolar bone tissues without the prior reduction and alkylation of disulfide bonds. The released peptides were then analyzed using liquid chromatography-tandem mass spectrometry. When 2-propanol was used in the pre-incubation step, this approach identified 487 proteins in the maxillary (upper jaw) bone and 443 proteins in the mandibular (lower jaw) bone, 40 of each being specifically ascribed to bone tissue. This suggests that routine use of our technique can provide deeper insights into the field of oral surgery at the molecular level. Furthermore, it could also have diagnostic potential in differentiating between the proteomic patterns of healthy and pathological alveolar bone tissue. The mass spectrometry proteomics data are available via ProteomeXchange with identifier PXD018836.

中文翻译：

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在骨组织中直接胰蛋白酶裂解，然后进行 LC-MS/MS 作为常规表征嵌入牙槽骨中的蛋白质的第一步

摘要 虽然放射学或组织学等方法经常用于口腔外科手术，但缺少一种在分子水平上表征牙槽骨的简单有效的方法。这主要是因为硬组织的蛋白质组学分析是一项严峻的分析挑战。在这项研究中，我们开发了一种简单的技术，用于口腔外科领域骨组织的蛋白质组学表征。在用不同溶剂进行预洗步骤后，直接在猪牙槽骨组织中用胰蛋白酶进行特异性消化，无需事先将二硫键还原和烷基化。然后使用液相色谱-串联质谱法分析释放的肽。当在预孵育步骤中使用 2-丙醇时，这种方法在上颌骨（上颌骨）中鉴定出 487 种蛋白质，在下颌骨（下颌骨）中鉴定出 443 种蛋白质，其中的 40 种蛋白质分别属于骨组织。这表明常规使用我们的技术可以在分子水平上对口腔外科领域提供更深入的了解。此外，它还可以在区分健康和病理性牙槽骨组织的蛋白质组学模式方面具有诊断潜力。质谱蛋白质组学数据可通过 ProteomeXchange 获得，标识符为 PXD018836。它还可以在区分健康和病理性牙槽骨组织的蛋白质组学模式方面具有诊断潜力。质谱蛋白质组学数据可通过 ProteomeXchange 获得，标识符为 PXD018836。它还可以在区分健康和病理性牙槽骨组织的蛋白质组学模式方面具有诊断潜力。质谱蛋白质组学数据可通过 ProteomeXchange 获得，标识符为 PXD018836。