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DNA synthesis increases during the first hours post-emergence in Anopheles albimanus mosquito midgut.
Developmental & Comparative Immunology ( IF 2.9 ) Pub Date : 2020-06-08 , DOI: 10.1016/j.dci.2020.103753
Krystal Maya-Maldonado 1 , Victor Cardoso-Jaime 1 , Salvador Hernández-Martínez 2 , Carlos Vázquez-Calzada 3 , Fidel de la Cruz Hernández-Hernández 3 , Humberto Lanz-Mendoza 2
Affiliation  

In hematophagous insects, the midgut is a fundamental barrier against infections and limits the development and transmission of pathogens. However, in mosquitoes, cell differentiation, proliferation, and cell cycle process in the midgut have not been characterized. Here we provide evidence of how cell cycle progression occurs in the newly emerged Anopheles albimanus mosquito midgut and describing cyclins expression as mediators of the cell cycle. The cell cycle at different post-emergence times was evaluated in disaggregated cells from midgut tissue using flow cytometry. Also, cyclins A, B, and E were identified by bioinformatics tools. These cyclins were used to analyze cell cycle progression. Flow cytometry data and the expression-pattern of the cyclins by qRT-PCR supported a polyploidy process, besides mitosis marker was marginally detected and only in newly emerged mosquitoes. Our results suggest that DNA increment in midguts occurs by polyploidy during the first hours post-emergence.



中文翻译:

在白化按蚊蚊子中肠出现后的最初几个小时内,DNA 合成增加。

在食血昆虫中,中肠是抵御感染的基本屏障,并限制病原体的发展和传播。然而,在蚊子中,中肠中的细胞分化、增殖和细胞周期过程尚未得到表征。在这里,我们提供了新出现的白色按蚊中细胞周期进程如何发生的证据蚊子中肠并将细胞周期蛋白的表达描述为细胞周期的介质。使用流式细胞术在来自中肠组织的解聚细胞中评估不同出现后时间的细胞周期。此外,细胞周期蛋白 A、B 和 E 由生物信息学工具识别。这些细胞周期蛋白用于分析细胞周期进程。流式细胞术数据和 qRT-PCR 的细胞周期蛋白表达模式支持多倍体过程,除了边缘检测到有丝分裂标记外,仅在新出现的蚊子中检测到。我们的结果表明,在出苗后的最初几个小时内,中肠中的 DNA 增加是通过多倍体发生的。

更新日期:2020-06-08
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