Circulating tumor cells (CTCs) represent the most common way of tumor metastasis and has been considered as a significant index for tumor diagnosis, staging and prognosis. However, CTC detection and analysis are always limited by the scarcity of CTC in the peripheral blood and the interference of blood cells. Therefore, here we presented with a hydrogen peroxide (H₂O₂)–response nanoprobes with CD44-targeted ability to reduce the interference of blood cells and improve the detection efficiency and accuracy and the pancreatic cancer cell was used to evaluate the feasibility of our probe. Shortly, hydrophobic H₂O₂-response naphthalimide-borate fluorophore was introduced onto the hydrophilic hyaluronic acid to form an amphiphilic complex, which could self-assemble into fluorescent nanoprobes in water. Our studies demonstrated that the nanoprobes were not only able to specifically recognize the pancreatic cancer cells with overexpressed CD44 proteins and reduce the influence of white blood cells in the peripheral blood, but also capable of semi-quantifying H₂O₂ content in CTCs, Which could be further used as a significant index for tumor clinical evaluation and therapy.

循环肿瘤细胞（CTC）代表了最常见的肿瘤转移方式，并被认为是肿瘤诊断，分期和预后的重要指标。然而，CTC的检测和分析总是受到外周血中CTC缺乏和血细胞干扰的限制。因此，在这里，我们提出了一种具有过氧化氢（H ₂ O ₂）响应能力的纳米探针，该探针具有CD44靶向能力，能够减少血细胞的干扰并提高检测效率和准确性，并使用胰腺癌细胞来评估我们的可行性探测。很快，疏水性H ₂ O ₂将响应的萘二甲酰亚胺硼酸酯荧光团引入亲水性透明质酸中，形成两亲性配合物，该配合物可在水中自组装成荧光纳米探针。我们的研究表明，纳米探针不仅能够特异性识别具有过表达CD44蛋白的胰腺癌细胞并减少外周血中白细胞的影响，而且还能够半定量CTC中的H ₂ O ₂含量。可以进一步用作肿瘤临床评估和治疗的重要指标。