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The complete chloroplast genome and characteristics analysis of Callistemon rigidus R.Br.
Molecular Biology Reports ( IF 2.8 ) Pub Date : 2020-06-08 , DOI: 10.1007/s11033-020-05567-4
Fenxiang Liu 1 , Ali Movahedi 2 , Wenguo Yang 3 , Lei Xu 4 , Jigang Xie 1 , Yu Zhang 1
Affiliation  

Callistemon rigidus R.Br. one of the traditional Chinese medicinal plants, is acrid-flavored and mild-natured, with the prominent effects reducing swelling, resolving phlegm, and dispelling rheumatism. Clinically, it has been commonly used to treat cold, cough and asthma, pain and swelling from impact injuries, eczema, rheumatic arthralgia. The chloroplast genome study on Callistemon rigidus R.Br. is a few seen. This study demonstrates the data collected from the assembly and annotation of the chloroplast (cp) genome of Callistemon rigidus R.Br., followed by furthers comparative analysis with the cp genomes of closely related species. C. rigidus R.Br. showed a cp genome in the size of 158, 961 bp long with 36.78% GC content, among which a pair of inverted repeats (IRs) of 26, 671 bp separated a large single-copy (LSC) region of 87, 162 bp and a small single-copy (SSC) region of 18, 457 bp. Altogether 131 genes were hosted, including 37 transfer RNAs, 8 ribosomal RNAs, and 86 protein-coding genes. 284 simple sequence repeats (SSRs) were also marked out. A comparative analysis of the genome structure and the sequence data of closely related species unveiled the conserved gene order in the IR and LSC/SSC regions, a quite constructive finding for future phylogenetic research. Overall, this study providing C. rigidus R.Br. genomic resources could positively contribute to the evolutionary study and the phylogenetic reconstruction of Myrtaceae.



中文翻译:

硬叶Callistemon R.Br.的完整叶绿体基因组和特征分析。

硬皮Callistemon s R.Br. 一种传统的中草药植物,具有辛辣和温和的特性,具有显着的功效,可减轻肿胀,化痰和祛风湿。临床上,它通常用于治疗感冒,咳嗽和哮喘,撞击伤,湿疹,风湿性关节痛引起的疼痛和肿胀。僵硬Callistemon s R.Br.的叶绿体基因组研究。看到了一些。这项研究证明了从Callistemon hardu s R.Br.的叶绿体(cp)基因组的组装和注释中收集的数据,然后进一步对紧密相关物种的cp基因组进行了比较分析。僵硬梭菌布朗 展示了一个cp基因组,长158,961 bp,GC含量为36.78%,其中一对26、671 bp的反向重复(IR)分隔了一个大的单拷贝(LSC)区域,分别为87、162 bp和18 457 bp的小单拷贝(SSC)区域。共托管了131个基因,包括37个转移RNA,8个核糖体RNA和86个蛋白质编码基因。还标记了284个简单序列重复(SSR)。对紧密相关物种的基因组结构和序列数据进行的比较分析揭示了IR和LSC / SSC区域的保守基因顺序,这对于将来的系统发育研究具有相当建设性的发现。总的来说,这项研究提供了僵硬梭菌R.Br。基因组资源可以为桃金娘科的进化研究和系统发育重建做出积极的贡献。

更新日期:2020-06-08
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