Formaldehyde has been widely used as an anticontaminant in artificial diets for rearing insects. However, the substance is highly toxic to humans. The objective of this work was to verify if replacing formaldehyde by acetylsalicylic acid, sorbic acid, or benzoic acid in artificial diet to use for rearing Diatraea saccharalis affect biological traits of its parasitoids Trichogramma galloi and Cotesia flavipes produced in this host species. The experimental design was the completely randomized with the following treatments (dose/diet): acetylsalicylic acid (3.7 g), sorbic acid (3.7 g), benzoic acid (3.7 g), formaldehyde (1.7 ml), and control. The studies of the biological characteristics of T. galloi and C. flavipes consisted of 25 replicates. For T. galloi, each replicate consisted of a mass of 30 eggs of D. saccharalis that were exposed for 24 h to a T. galloi female which was less than 24 h old. For C. flavipes , each replicate used a third instar D. saccharalis caterpillar oviposited by a C. flavipes female less than 24 h old. The biological characteristics evaluated were parasitism, emergence, progeny, sexual ratio and longevity. The use of alternative anticontaminants tested in the artificial diets of D. saccharalis is feasible for the production of T. galloi and C. flavipes parasitoids.

中文翻译：

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在用于饲养甘蔗螟的人工饲料中替代甲醛的抗污染物剂会影响寄生蜂的性能吗？

甲醛已被广泛用作饲养昆虫的人工饲料中的抗污染剂。然而，该物质对人体有剧毒。这项工作的目的是验证在人工饲料中用乙酰水杨酸、山梨酸或苯甲酸代替甲醛以用于饲养 Diatraea saccharalis 是否会影响其寄生蜂 Trichogramma galloi 和 Cotesia flavipes 在该宿主物种中产生的生物学特性。实验设计是完全随机化的，具有以下处理（剂量/饮食）：乙酰水杨酸（3.7 g）、山梨酸（3.7 g）、苯甲酸（3.7 g）、甲醛（1.7 ml）和对照。T. galloi 和 C. flavipes 的生物学特性研究包括 25 个重复。对于 T. galloi，每个重复由 30 个 D 卵组成。saccharalis 暴露于小于 24 小时的 T. galloi 雌性 24 小时。对于 C. flavipes，每个重复使用由小于 24 小时的 C. flavipes 雌性产卵的三龄 D. saccharalis 毛虫。评估的生物学特性是寄生性、羽化、后代、性比和寿命。在 D. saccharalis 的人工饲料中测试的替代抗污染物的使用对于 T. galloi 和 C. flavipes 寄生蜂的生产是可行的。