Endo-1,4-β-mannosidases catalyze the cleavage of the β-d-1,4-mannopyranosyl linkage in mannan and have potential biotechnological applications in biofuel production, manno-oligosaccharide production, cleansing, and food and feed industries. In this study, an endo-1,4-β-mannosidase gene (TaMan3A) from wheat (Triticum aestivum) was successfully expressed in Pichia pastoris, and the antifungal effectiveness and manno-oligosaccharide production of the enzyme were evaluated. The purified TaMan3A exhibited a molecular weight of approximately 43 kDa, and its highest enzyme activity at pH 4.0 and 40 °C. Comparisons with other β-mannanases showed that TaMan3A had a conserved mannan-binding V-shaped groove, catalytic acid/base residue (E179), and nucleophilic residue (E297). Antifungal assays for TaMan3A against seven fungi commonly associated with wheat kernels showed that this enzyme had higher inhibitory effects on hyphal growth of the field fungi Fusarium graminearum and Alternaria sp. in comparison with that of storage fungi. TaMan3A could hydrolyze mannan polymers of galactomannan and Konjac glucomannan to mannobiose, mannotriose, and mannotetraose as the main products. Overall, these results showed the potential of TaMan3A for enhancing host resistance against fungal pathogens in wheat and manno-oligosaccharide production in the feed and food industries.

Endo-1,4- β-甘露糖苷酶催化甘露聚糖中β- d -1,4-甘露吡喃糖基键的裂解，在生物燃料生产，甘露寡糖生产，清洁以及食品和饲料工业中具有潜在的生物技术应用。在这项研究中，成功地在毕赤酵母中表达了小麦（Triticum aestivum）的内切1,4- β-甘露糖苷酶基因（TaMan 3A），并评估了该酶的抗真菌作用和甘露寡糖的产生。纯化的Ta Man3A的分子量约为43 kDa，在pH 4.0和40°C时具有最高的酶活性。与其他β-甘露聚糖酶的比较表明Ta Man3A具有保守的甘露聚糖结合V型槽，催化酸/碱残基（E179）和亲核残基（E297）。Ta Man3A对7种通常与小麦粒相关的真菌的抗真菌试验表明，该酶对田间镰刀镰刀菌和链格孢菌的菌丝生长具有较高的抑制作用。与储存真菌相比。Ta Man3A可以将半乳甘露聚糖和魔芋葡甘露聚糖的甘露聚糖聚合物水解为甘露二糖，甘露三糖和甘露糖。总体而言，这些结果表明了钽的潜力Man3A用于增强宿主对饲料和食品工业中小麦和甘露寡糖生产中真菌病原体的抗性。