Objective: This study aimed to detect hepatic oxidative damage caused by aflatoxin B1 (AFB1), as well as to examine how propolis protects against hepatotoxic effects of AFB1.

Methods: Rats were split into four groups as control group, AFB1 group, propolis group, AFB1+ propolis group.

Results: There was significant increase in malondialdehyde (MDA) level and tumor suppressor protein (TP53) gene expression, Glutathione (GSH) level, Catalase (CAT) activity, CAT gene expression decreased in AFB1 group in blood. MDA level and Glutathione-S-Transferase (GST) activity, GST and TP53 gene expressions increased in AFB1 group, whereas GSH level and CAT activity alongside CAT gene expression decreased in liver. AFB1+propolis group showed significant decrease in MDA level, GST activity, TP53 and GST gene expressions, GSH level and CAT activity and CAT gene expression increased in liver compared to AFB1 group.

Conclusion: These results suggest that propolis may potentially be natural agent that prevents AFB1- induced oxidative stress and hepatotoxicity.

目的：本研究旨在检测由黄曲霉毒素B1（AFB1）引起的肝氧化损伤，以及蜂胶如何保护AFB1免受肝毒性作用。

方法：将大鼠分为对照组，AFB1组，蜂胶组，AFB1 +蜂胶组四组。

结果：血液中AFB1组的丙二醛（MDA）和抑癌蛋白（TP53）基因表达，谷胱甘肽（GSH）水平，过氧化氢酶（CAT）活性，CAT基因表达显着增加。AFB1组MDA水平和谷胱甘肽-S-转移酶（GST）活性，GST和TP53基因表达增加，而肝脏中GSH水平和CAT活性以及CAT基因表达下降。与AFB1组相比，AFB1 +蜂胶组的肝脏中MDA水平，GST活性，TP53和GST基因表达，GSH水平，CAT活性和CAT基因表达显着降低。

结论：这些结果表明蜂胶可能是预防AFB1诱导的氧化应激和肝毒性的天然药物。