Background: Tea is one of the most popular calming drinks. Drought is a major environmental factor that limited the growth and development of plants.

Objective: Therefore, the identification of proteins under the drought stress conditions in tea can have an essential role in the breeding programs and tea production.

Methods: For this purpose, 14 tea clones were studied under normal and drought stress conditions in two separate experiments, and the leaves of the clones were stored at −80°C. After the identification of two clones (100 and 278) as tolerant and sensitive clones, respectively, the proteomics approach was used to compare the leaf protein profile changes under both conditions.

Results: The results of proteomics showed about 500 detectable protein spots, of which 250 spots were repeatable. Among the 250 reproducible spots, 16 spots responded to the drought stress, which showed the highest amount of variation among the treatments. Thioredoxin, peroxidase, superoxide dismutase, ribosomal protein, and hsp70 were mentioned among the identified proteins. These proteins were involved in various cellular functions.

Conclusion: Identified proteins also had a crucial role in regulating carbohydrate and nitrogen metabolism and the scavenging of the Reactive Oxygen Species (ROS). Upregulation of proteins involved in protein processing (ribosomal protein), oxygen species scavenging, and defense (Superoxide dismutase, Peroxidase, and thioredoxin) may increase plant adaptation to drought stress. This study was the first report that showed ribosomal protein L32 was significantly changed in tea against drought stress response. Therefore, these proteins can protect the plant against drought stress. This study partially identified the drought stress proteins in the tea plant.

背景：茶是最受欢迎的镇静饮料之一。干旱是限制植物生长发育的主要环境因素。

目的：因此，茶中干旱胁迫条件下蛋白质的鉴定对育种程序和茶的生产具有重要作用。

方法：为此目的，在两个单独的实验中在正常和干旱胁迫条件下研究了14个茶树无性系，并将它们的叶片保存在-80°C下。在将两个克隆（100和278）分别鉴定为耐受和敏感克隆后，使用蛋白质组学方法比较了两种条件下的叶片蛋白质谱变化。

结果：蛋白质组学结果显示可检测到约500个蛋白斑点，其中250个斑点可重复。在250个可重现的斑点中，有16个斑点对干旱胁迫有反应，这表明在处理之间差异最大。在鉴定出的蛋白质中提到了硫氧还蛋白，过氧化物酶，超氧化物歧化酶，核糖体蛋白和hsp70。这些蛋白质参与各种细胞功能。

结论：鉴定出的蛋白质在调节碳水化合物和氮的代谢以及清除活性氧（ROS）中也起着关键作用。参与蛋白质加工（核糖体蛋白），清除氧和防御（超氧化物歧化酶，过氧化物酶和硫氧还蛋白）的蛋白质上调可能会增强植物对干旱胁迫的适应性。这项研究是第一份报告，显示茶核糖体蛋白L32在抗干旱胁迫反应中发生了显着变化。因此，这些蛋白质可以保护植物免受干旱胁迫。这项研究部分鉴定了茶树中的干旱胁迫蛋白。